catalog number :
MBS749305
products type :
ELISA Kit
products full name :
Bovine Pregnanediol-3-Glucuronide ELISA Kit
products short name :
Pregnanediol-3-Glucuronide
specificity :
This assay has high sensitivity and excellent specificity for detection of P3GLU. No significant cross-reactivity or interference between P3GLU and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between P3GLU and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 ng/mL.
other info2 :
Intended Uses: This P3GLU ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine P3GLU. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
products description :
Priciple of the assay: P3GLU ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-P3GLU antibody and an P3GLU-HRP conjugate. The assay sample and buffer are incubated together with P3GLU-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the P3GLU concentration since P3GLU from samples and P3GLU-HRP conjugate compete for the anti-P3GLU antibody binding site. Since the number of sites is limited, as more sites are occupied by P3GLU from the sample, fewer sites are left to bind P3GLU-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The P3GLU concentration in each sample is interpolated from this standard curve.