Monkey Growth-regulated nocogenea/melanoma growth stimulating ELISA Kit | MyBioSource MBS748962 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Monkey Growth-regulated nocogenea/melanoma growth stimulating ELISA Kit

catalog :

MBS748962

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS748962

products type :

ELISA Kit

products full name :

Monkey Growth-regulated nocogenea/melanoma growth stimulating ELISA Kit

products short name :

Growth-regulated nocogenea/melanoma growth stimulating

other names :

growth-regulated alpha protein; Growth-regulated alpha protein; growth-regulated alpha protein; MGSA alpha; GRO-alpha(1-73); C-X-C motif chemokine 1; fibroblast secretory protein; neutrophil-activating protein 3; melanoma growth stimulatory activity alpha; GRO1 oncogene (melanoma growth-stimulating activity); GRO1 oncogene (melanoma growth stimulating activity, alpha); chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha); C-X-C motif chemokine 1; GRO-alpha(1-73); Melanoma growth stimulatory activity; MGSA; Neutrophil-activating protein 3; NAP-3

products gene name :

GROa/MGSA

other gene names :

CXCL1; CXCL1; FSP; GRO1; GROa; MGSA; NAP-3; SCYB1; MGSA-a; GRO; GRO1; GROA; MGSA; SCYB1; MGSA; NAP-3

uniprot entry name :

GROA_HUMAN

reactivity :

Monkey

specificity :

This assay has high sensitivity and excellent specificity for detection of GRN. No significant cross-reactivity or interference between GRN and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GRN and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 0.1 ng/mL.

other info2 :

Intended Uses: This GRN ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey GRN. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products description :

Principle of the assay: GRN ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for GRN. Standards or samples are then added to the microtiter plate wells and GRN if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of GRN present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for GRN are added to each well to "sandwich" the GRN immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain GRN and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GRN concentration in each sample is interpolated from this standard curve.

ncbi gi num :

4504153

ncbi acc num :

NP_001502.1

ncbi gb acc num :

NM_001511.3

ncbi mol weight :

11,301 Da

ncbi pathways :

Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Chemokine Receptors Bind Chemokines Pathway (106359); Chemokine Signaling Pathway (99051); Chemokine Signaling Pathway (96864); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Cytokines And Inflammatory Response Pathway (198794); Epithelial Cell Signaling In Helicobacter Pylori Infection Pathway (83102)

ncbi summary :

This antimicrobial gene encodes a member of the CXC subfamily of chemokines. The encoded protein is a secreted growth factor that signals through the G-protein coupled receptor, CXC receptor 2. This protein plays a role in inflammation and as a chemoattractant for neutrophils. Aberrant expression of this protein is associated with the growth and progression of certain tumors. A naturally occurring processed form of this protein has increased chemotactic activity. Alternate splicing results in coding and non-coding variants of this gene. A pseudogene of this gene is found on chromosome 4. [provided by RefSeq, Sep 2014]

uniprot summary :

CXCL1: Has chemotactic activity for neutrophils. May play a role in inflammation and exerts its effects on endothelial cells in an autocrine fashion. In vitro, the processed forms GRO- alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity. Belongs to the intercrine alpha (chemokine CxC) family. Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 4q21. Cellular Component: extracellular space; extracellular region. Molecular Function: growth factor activity; chemokine activity; CXCR chemokine receptor binding; enzyme activator activity; receptor binding. Biological Process: positive regulation of catalytic activity; nervous system development; negative regulation of cell proliferation; cell proliferation; G-protein coupled receptor protein signaling pathway; response to lipopolysaccharide; immune response; positive regulation of leukocyte chemotaxis; signal transduction; inflammatory response; actin cytoskeleton organization and biogenesis; chemotaxis

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage