ELISA/assay

Mouse Steroidogenic acute regulatory protein ELISA Kit

MBS748608

48-Strip-Wells

470 USD

ELISA Kit

Mouse Steroidogenic acute regulatory protein ELISA Kit

Steroidogenic acute regulatory protein

steroidogenic acute regulatory protein; Steroidogenic acute regulatory protein, mitochondrial; steroidogenic acute regulatory protein, mitochondrial; cholesterol trafficker; START domain containing 1; steroid acute regulatory protein; START domain-containing protein 1; mitochondrial steroid acute regulatory protein; StAR-related lipid transfer (START) domain containing 1; steroidogenic acute regulatory protein; START domain-containing protein 1; StARD1

StAR

STAR; STAR; STARD1; STARD1; StAR; StARD1

STAR_HUMAN

Mouse

This assay has high sensitivity and excellent specificity for detection of STAR. No significant cross-reactivity or interference between STAR and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between STAR and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This STAR ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse STAR. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Signal Transduction

Principle of the assay: STAR ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-STAR antibody and an STAR-HRP conjugate. The assay sample and buffer are incubated together with STAR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the STAR concentration since STAR from samples and STAR-HRP conjugate compete for the anti-STAR antibody binding site. Since the number of sites is limited, as more sites are occupied by STAR from the sample, fewer sites are left to bind STAR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The STAR concentration in each sample is interpolated from this standard curve.

2661026

AAB88174.1

31,914 Da

Corticotropin-releasing Hormone Pathway (920957); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); Metabolism Of Steroid Hormones And Vitamin D Pathway (106150); Ovarian Steroidogenesis Pathway (790011); Ovarian Steroidogenesis Pathway (795173); Pregnenolone Biosynthesis Pathway (106151)

The protein encoded by this gene plays a key role in the acute regulation of steroid hormone synthesis by enhancing the conversion of cholesterol into pregnenolone. This protein permits the cleavage of cholesterol into pregnenolone by mediating the transport of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane. Mutations in this gene are a cause of congenital lipoid adrenal hyperplasia (CLAH), also called lipoid CAH. A pseudogene of this gene is located on chromosome 13. [provided by RefSeq, Jul 2008]

STAR: Plays a key role in steroid hormone synthesis by enhancing the metabolism of cholesterol into pregnenolone. Mediates the transfer of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane where it is cleaved to pregnenolone. May interact with TSPO. Expressed in gonads, adrenal cortex and kidney. Protein type: Carrier; Mitochondrial; Lipid-binding. Chromosomal Location of Human Ortholog: 8p11.2. Cellular Component: mitochondrial crista; neuron projection; cell soma; mitochondrial intermembrane space; cytosol. Molecular Function: cholesterol transporter activity; cholesterol binding. Biological Process: response to nicotine; steroid metabolic process; diterpenoid metabolic process; dibenzo-p-dioxin metabolic process; regulation of steroid biosynthetic process; estrogen biosynthetic process; biphenyl metabolic process; circadian sleep/wake cycle, REM sleep; negative regulation of neuron apoptosis; response to nutrient; response to corticosterone stimulus; response to drug; cholesterol metabolic process; bile acid biosynthetic process; progesterone biosynthetic process; positive regulation of neurogenesis; insecticide metabolic process; fractalkine metabolic process; male gonad development; phthalate metabolic process; intracellular cholesterol transport; response to herbicide; glucocorticoid metabolic process; response to ethanol; cellular response to insulin stimulus; response to hydrogen peroxide; response to estrogen stimulus; response to lead ion; C21-steroid hormone biosynthetic process; brain development; response to ionizing radiation; response to activity; steroid biosynthetic process; regulation of neuronal synaptic plasticity; phenol metabolic process. Disease: Lipoid Congenital Adrenal Hyperplasia

48-Strip-Wells

470 USD

96-Strip-Wells

675