catalog number :
MBS748477
products type :
ELISA Kit
products full name :
Human Anti Ganglioside M2 Antibody ELISA Kit
products short name :
Anti Ganglioside M2 Antibody
specificity :
This assay has high sensitivity and excellent specificity for detection of GM2Ab. No significant cross-reactivity or interference between GM2Ab and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GM2Ab and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 ng/mL.
other info2 :
Intended Uses: This GM2Ab ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human GM2Ab. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
products categories :
Cell Biology
products description :
Principle of the Assay: GM2Ab ELISA kit applies the competitive enzyme immunoassay technique utilizing Ganglioside M2 antigen and an GM2Ab-HRP conjugate. The assay sample and buffer are incubated together with GM2Ab-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GM2Ab concentration since GM2Ab from samples and GM2Ab-HRP conjugate compete for the Ganglioside M2 antigen binding site. Since the number of sites is limited, as more sites are occupied by GM2Ab from the sample, fewer sites are left to bind GM2Ab-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GM2Ab concentration in each sample is interpolated from this standard curve.