ELISA/assay

Porcine Acyl Ghrelin ELISA Kit

MBS748187

48-Strip-Wells

470 USD

ELISA Kit

Porcine Acyl Ghrelin ELISA Kit

Acyl Ghrelin

ghrelin; Ghrelin; Ghrelin

A-GHR

E7CCH4_HUMAN

Porcine

This assay has high sensitivity and excellent specificity for detection of AGHRL. No significant cross-reactivity or interference between AGHRL and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between AGHRL and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This AGHRL ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine AGHRL. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the assay: AGHRL ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AGHRL antibody and an AGHRL-HRP conjugate. The assay sample and buffer are incubated together with AGHRL-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AGHRL concentration since AGHRL from samples and AGHRL-HRP conjugate compete for the anti-AGHRL antibody binding site. Since the number of sites is limited, as more sites are occupied by AGHRL from the sample, fewer sites are left to bind AGHRL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AGHRL concentration in each sample is interpolated from this standard curve.

304435649

ADM33790.1

12,992 Da

This gene encodes the ghrelin-obestatin preproprotein that is cleaved to yield two peptides, ghrelin and obestatin. Ghrelin is a powerful appetite stimulant and plays an important role in energy homeostasis. Its secretion is initiated when the stomach is empty, whereupon it binds to the growth hormone secretagogue receptor in the hypothalamus which results in the secretion of growth hormone (somatotropin). Ghrelin is thought to regulate multiple activities, including hunger, reward perception via the mesolimbic pathway, gastric acid secretion, gastrointestinal motility, and pancreatic glucose-stimulated insulin secretion. It was initially proposed that obestatin plays an opposing role to ghrelin by promoting satiety and thus decreasing food intake, but this action is still debated. Recent reports suggest multiple metabolic roles for obestatin, including regulating adipocyte function and glucose metabolism. Alternative splicing results in multiple transcript variants. In addition, antisense transcripts for this gene have been identified and may potentially regulate ghrelin-obestatin preproprotein expression. [provided by RefSeq, Nov 2014]

48-Strip-Wells

470 USD

96-Strip-Wells

675