ELISA/assay

Rat Transient receptor potential cation channel, subfamily V, member 1 ELISA Kit

MBS748108

48-Strip-Wells

470 USD

ELISA Kit

Rat Transient receptor potential cation channel, subfamily V, member 1 ELISA Kit

Transient receptor potential cation channel, subfamily V, member 1

transient receptor potential cation channel subfamily V member 1; Transient receptor potential cation channel subfamily V member 1; transient receptor potential cation channel subfamily V member 1; OTRPC1; capsaicin receptor; osm-9-like TRP channel 1; vanilloid receptor subtype 1; transient receptor potential vanilloid 1a; transient receptor potential vanilloid 1b; transient receptor potential cation channel, subfamily V, member 1; Capsaicin receptor; Osm-9-like TRP channel 1; OTRPC1; Vanilloid receptor 1

TRPV1

TRPV1; TRPV1; VR1; VR1; TrpV1; OTRPC1

TRPV1_HUMAN

Rat

This assay has high sensitivity and excellent specificity for detection of TRPV-1. No significant cross-reactivity or interference between TRPV-1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between TRPV-1 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This TRPV-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat TRPV-1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Signal Transduction

Principle of the assay: TRPV-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TRPV-1 antibody and an TRPV-1-HRP conjugate. The assay sample and buffer are incubated together with TRPV-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TRPV-1 concentration since TRPV-1 from samples and TRPV-1-HRP conjugate compete for the anti-TRPV-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by TRPV-1 from the sample, fewer sites are left to bind TRPV-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TRPV-1 concentration in each sample is interpolated from this standard curve.

74315354

NP_542437.2

NM_080706.3

94,956 Da

Inflammatory Mediator Regulation Of TRP Channels Pathway (948277); Inflammatory Mediator Regulation Of TRP Channels Pathway (948307); Ion Channel Transport Pathway (187193); Neuroactive Ligand-receptor Interaction Pathway (83053); Neuroactive Ligand-receptor Interaction Pathway (462); Stimuli-sensing Channels Pathway (771568); TRP Channels Pathway (906028); Transmembrane Transport Of Small Molecules Pathway (106572); Trk Receptor Signaling Mediated By PI3K And PLC-gamma Pathway (137967); Trk Receptor Signaling Mediated By The MAPK Pathway (138044)

Capsaicin, the main pungent ingredient in hot chili peppers, elicits a sensation of burning pain by selectively activating sensory neurons that convey information about noxious stimuli to the central nervous system. The protein encoded by this gene is a receptor for capsaicin and is a non-selective cation channel that is structurally related to members of the TRP family of ion channels. This receptor is also activated by increases in temperature in the noxious range, suggesting that it functions as a transducer of painful thermal stimuli in vivo. Four transcript variants encoding the same protein, but with different 5' UTR sequence, have been described for this gene. [provided by RefSeq, Jul 2008]

TRPV1: a receptor for capsaicin and a non-selective cation channel that is structurally related to members of the TRP family of ion channels. Activated by increases in temperature in the noxious range, suggesting that it functions as a transducer of painful thermal stimuli in vivo. Protein type: Channel, cation; Receptor, misc.; Membrane protein, integral; Membrane protein, multi-pass. Chromosomal Location of Human Ortholog: 17p13.2. Cellular Component: postsynaptic membrane; integral to plasma membrane; integral to membrane; plasma membrane; cell junction; intrinsic to plasma membrane. Molecular Function: calmodulin binding; transmembrane receptor activity; excitatory extracellular ligand-gated ion channel activity; calcium channel activity; calcium-release channel activity; phosphoprotein binding; ATP binding. Biological Process: cell surface receptor linked signal transduction; transport; chemosensory behavior; thermoception; transmembrane transport

48-Strip-Wells

470 USD

96-Strip-Wells

675