ELISA/assay

Mouse Insulin receptor substrate 1 ELISA Kit

MBS748070

48-Strip-Wells

470 USD

ELISA Kit

Mouse Insulin receptor substrate 1 ELISA Kit

Insulin receptor substrate 1

insulin receptor substrate 1; Insulin receptor substrate 1; insulin receptor substrate 1; IRS-1; insulin receptor substrate 1

IRS-1

IRS1; IRS1; HIRS-1; IRS-1

IRS1_HUMAN

Mouse

This assay has high sensitivity and excellent specificity for detection of IRS1. No significant cross-reactivity or interference between IRS1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IRS1 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This IRS1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse IRS1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the assay: IRS1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IRS1 antibody and an IRS1-HRP conjugate. The assay sample and buffer are incubated together with IRS1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IRS1 concentration since IRS1 from samples and IRS1-HRP conjugate compete for the anti-IRS1 antibody binding site. Since the number of sites is limited, as more sites are occupied by IRS1 from the sample, fewer sites are left to bind IRS1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IRS1 concentration in each sample is interpolated from this standard curve.

5031805

NP_005535.1

NM_005544.2

131,591 Da

AGE/RAGE Pathway (698754); AMPK Signaling Pathway (989139); AMPK Signaling Pathway (992181); Adaptive Immune System Pathway (366160); Adipocytokine Signaling Pathway (83093); Adipocytokine Signaling Pathway (505); Adipogenesis Pathway (198832); Aldosterone-regulated Sodium Reabsorption Pathway (130626); Aldosterone-regulated Sodium Reabsorption Pathway (130590); Alpha6-Beta4 Integrin Signaling Pathway (198807)

This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance. [provided by RefSeq, Nov 2009]

IRS1: an adaptor protein that is one of the major substrates of the insulin receptor kinase. Contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2-domain-containing proteins including phosphatidylinositol 3-kinase p85 subunit and GRB-2. Phosphorylated on ser/thr residues by kinases including JNK and IKK. Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 2q36. Cellular Component: intracellular membrane-bound organelle; cytoplasm; plasma membrane; caveola; nucleus; cytosol. Molecular Function: insulin-like growth factor receptor binding; protein binding; signal transducer activity; protein kinase C binding; phosphoinositide 3-kinase binding; SH2 domain binding; transmembrane receptor protein tyrosine kinase adaptor protein activity; insulin receptor binding. Biological Process: response to peptide hormone stimulus; phosphoinositide 3-kinase cascade; nerve growth factor receptor signaling pathway; positive regulation of glycogen biosynthetic process; negative regulation of insulin receptor signaling pathway; glucose homeostasis; signal transduction; negative regulation of insulin secretion; response to insulin stimulus; positive regulation of glucose import; mammary gland development; positive regulation of mesenchymal cell proliferation; positive regulation of cell proliferation; protein kinase B signaling cascade; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; positive regulation of phosphoinositide 3-kinase activity; positive regulation of fatty acid beta-oxidation; cellular response to insulin stimulus; insulin-like growth factor receptor signaling pathway; regulation of gene expression; insulin receptor signaling pathway; innate immune response; positive regulation of insulin receptor signaling pathway; lipid catabolic process; positive regulation of cell migration. Disease: Diabetes Mellitus, Noninsulin-dependent

48-Strip-Wells

470 USD

96-Strip-Wells

675