ELISA/assay

Rabbit Lipoxin A4 ELISA Kit

MBS748043

48-Strip-Wells

470 USD

ELISA Kit

Rabbit Lipoxin A4 ELISA Kit

Lipoxin A4

lipoxin A4 receptor; N-formyl peptide receptor 2; N-formyl peptide receptor 2; RFP; FMLP-R-I; LXA4 receptor; FMLP-related receptor I; formyl peptide receptor-like 1; lipoxin A4 receptor (formyl peptide receptor related); formyl peptide receptor 2; FMLP-related receptor I; FMLP-R-I; Formyl peptide receptor-like 1; HM63; Lipoxin A4 receptor; LXA4 receptor; RFP

LXA4

FPR2; FPR2; ALXR; HM63; FMLPX; FPR2A; FPRH1; FPRH2; FPRL1; LXA4R; FMLP-R-II; FPRH1; FPRL1; LXA4R; FMLP-R-I; LXA4 receptor

FPR2_HUMAN

Rabbit

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive

Intended Uses: This LXA4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit LXA4. This ELISA kit for research use only, not for therapeutic or diagnostic applications. !Intended Uses: This LXA4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit LXA4. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: LXA4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-LXA4 antibody and an LXA4-HRP conjugate. The assay sample and buffer are incubated together with LXA4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LXA4 concentration since LXA4 from samples and LXA4-HRP conjugate compete for the anti-LXA4 antibody binding site. Since the number of sites is limited, as more sites are occupied by LXA4 from the sample, fewer sites are left to bind LXA4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LXA4 concentration in each sample is interpolated from this standard curve.

1916076

AAB51133.1

38,964 Da

Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Formyl Peptide Receptors Bind Formyl Peptides And Many Other Ligands Pathway (161021); G Alpha (i) Signalling Events Pathway (119550); G Alpha (q) Signalling Events Pathway (106043); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); GPCRs, Class A Rhodopsin-like Pathway (198886); Gastrin-CREB Signalling Pathway Via PKC And MAPK (645295); Neuroactive Ligand-receptor Interaction Pathway (83053); Neuroactive Ligand-receptor Interaction Pathway (462)

FPR2: Low affinity receptor for N-formyl-methionyl peptides, which are powerful neutrophils chemotactic factors. Binding of FMLP to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. The activation of LXA4R could result in an anti-inflammatory outcome counteracting the actions of proinflammatory signals such as LTB4 (leukotriene B4). Belongs to the G-protein coupled receptor 1 family. Protein type: GPCR, family 1; Motility/polarity/chemotaxis; Membrane protein, integral; Receptor, GPCR; Membrane protein, multi-pass. Chromosomal Location of Human Ortholog: 19q13.3-q13.4. Cellular Component: plasma membrane; integral to membrane. Molecular Function: G-protein coupled receptor activity; N-formyl peptide receptor activity. Biological Process: G-protein coupled receptor protein signaling pathway; cell motility; inflammatory response; chemotaxis; cell adhesion

48-Strip-Wells

470 USD

96-Strip-Wells

675