ELISA/assay

Canine DNA Damage Inducible Transcript 3 ELISA Kit

MBS747949

48-Strip-Wells

470 USD

ELISA Kit

Canine DNA Damage Inducible Transcript 3 ELISA Kit

DNA Damage Inducible Transcript 3

DNA damage-inducible transcript 3 protein isoform 2; DNA damage-inducible transcript 3 protein; DNA damage-inducible transcript 3 protein; DDIT-3; C/EBP zeta; c/EBP-homologous protein 10; CCAAT/enhancer-binding protein homologous protein; growth arrest and DNA damage-inducible protein GADD153; DNA-damage-inducible transcript 3; C/EBP zeta; C/EBP-homologous protein; CHOP; C/EBP-homologous protein 10; CHOP-10; CCAAT/enhancer-binding protein homologous protein; Growth arrest and DNA damage-inducible protein GADD153

DDIT3

DDIT3; DDIT3; CHOP; CEBPZ; CHOP10; CHOP-10; GADD153; CHOP; CHOP10; GADD153; DDIT-3; CHOP; CHOP-10

DDIT3_HUMAN

Canine

This assay has high sensitivity and excellent specificity for detection of DDIT3. No significant cross-reactivity or interference between DDIT3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between DDIT3 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 1.0 pg/mL.

Intended Uses: This DDIT3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine DDIT3. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Cell Biology

Principle of the assay: DDIT3 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for DDIT3. Standards or samples are then added to the microtiter plate wells and DDIT3 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of DDIT3 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for DDIT3 are added to each well to “sandwich” the DDIT3 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain DDIT3 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DDIT3 concentration in each sample is interpolated from this standard curve.

304282234

NP_001181986.1

NM_001195057.1

21,700 Da

ATF-2 Transcription Factor Network Pathway (138006); ATF4 Activates Genes Pathway (530772); ATF6-alpha Activates Chaperone Genes Pathway (530770); ATF6-alpha Activates Chaperones Pathway (105905); Adipogenesis Pathway (198832); MAPK Signaling Pathway (198779); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Metabolism Of Proteins Pathway (106230); Non-alcoholic Fatty Liver Disease (NAFLD) Pathway (862188)

This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified. [provided by RefSeq, Aug 2010]

CHOP: a transcriptional-regulatory protein of the bZIP family. Inhibits the DNA-binding activity of C/EBP and LAP by forming heterodimers that cannot bind DNA. May play an important role in melanoma progression. CK2-mediated phosphorylation inhibits its transcriptional activity. Up-regulates IL-6 transcription by trapping negative regulating NF-IL6 isoform. Protein type: Oncoprotein; DNA-binding; Autophagy; Transcription factor. Chromosomal Location of Human Ortholog: 12q13.1-q13.2. Cellular Component: nucleoplasm; late endosome; nucleus; cytosol. Molecular Function: protein binding; leucine zipper domain binding; DNA binding; protein heterodimerization activity; transcription factor activity; transcription corepressor activity; transcription factor binding. Biological Process: proteasomal ubiquitin-dependent protein catabolic process; positive regulation of transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; positive regulation of interleukin-8 production; regulation of transcription, DNA-dependent; positive regulation of neuron apoptosis; cell cycle arrest; ER overload response; regulation of transcription in response to stress; Wnt receptor signaling pathway; negative regulation of protein kinase B signaling cascade; unfolded protein response; negative regulation of transcription factor activity; mRNA transcription from RNA polymerase II promoter; blood vessel maturation; response to unfolded protein; response to starvation; cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; cell redox homeostasis; release of sequestered calcium ion into cytosol; positive regulation of transcription from RNA polymerase II promoter; negative regulation of myoblast differentiation; negative regulation of transcription, DNA-dependent; inhibition of CREB transcription factor; response to DNA damage stimulus. Disease: Myxoid Liposarcoma

48-Strip-Wells

470 USD

96-Strip-Wells

675