ELISA/assay

Bovine Elastase 3B ELISA Kit

MBS747742

48-Strip-Wells

470 USD

ELISA Kit

Bovine Elastase 3B ELISA Kit

Elastase 3B

ELA3B protein; Chymotrypsin-like elastase family member 3B; chymotrypsin-like elastase family member 3B; elastase 1; protease E; elastase-3B; proteinase E; elastase IIIB; fecal elastase 1; pancreatic elastase 1; elastase 3B, pancreatic; pancreatic endopeptidase E; cholesterol-binding pancreatic protease; chymotrypsin-like elastase family, member 3B; Elastase IIIB; Elastase-3B; Protease E

ELA3B

CELA3B; CELA3B; E1; CBPP; EL-1; ELA3B; ELA3B

CEL3B_HUMAN

Bovine

This assay has high sensitivity and excellent specificity for detection of ELA3B. No significant cross-reactivity or interference between ELA3B and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ELA3B and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This ELA3B ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine ELA3B. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Cell Biology

Principle of the assay: ELA3B ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ELA3B antibody and an ELA3B-HRP conjugate. The assay sample and buffer are incubated together with ELA3B-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ELA3B concentration since ELA3B from samples and ELA3B-HRP conjugate compete for the anti-ELA3B antibody binding site. Since the number of sites is limited, as more sites are occupied by ELA3B from the sample, fewer sites are left to bind ELA3B-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ELA3B concentration in each sample is interpolated from this standard curve.

13528831

AAH05216.1

29,263 Da

Pancreatic Secretion Pathway (169306); Pancreatic Secretion Pathway (169295); Protein Digestion And Absorption Pathway (172847); Protein Digestion And Absorption Pathway (171868)

Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, elastase 3B has little elastolytic activity. Like most of the human elastases, elastase 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein, it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. Elastase 3B may also function in the intestinal transport and metabolism of cholesterol. Both elastase 3A and elastase 3B have been referred to as protease E and as elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function in clinical assays. [provided by RefSeq, May 2009]

CELA3B: Efficient protease with alanine specificity but only little elastolytic activity. Belongs to the peptidase S1 family. Elastase subfamily. Protein type: EC 3.4.21.70; Protease. Chromosomal Location of Human Ortholog: 1p36.12. Molecular Function: peptidase activity; serine-type endopeptidase activity. Biological Process: cholesterol metabolic process; proteolysis

48-Strip-Wells

470 USD

96-Strip-Wells

675