ELISA/assay

Mouse Beta-Defensin 14 ELISA Kit

MBS747419

48-Strip-Wells

440 USD

ELISA Kit

Mouse Beta-Defensin 14 ELISA Kit

[Beta-Defensin 14]

[Defb14 protein; Beta-defensin 14; beta-defensin 14; defensin beta 14; Defensin, beta 14]

[DEFb14]

[Defb14; Defb14; BD-14; BD-14; mBD-14]

DFB14_MOUSE

Mouse

This assay has high sensitivity and excellent specificity for detection of BDEF14. No significant cross-reactivity or interference between BDEF14 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between BDEF14 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Detection Range: 0.5-10ng/mL. Sensitivity: 0.1ng/mL

Intended Uses: This BDEF14 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse BDEF14. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Neurobiology

. Principle of the assay: BDEF14 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-BDEF14 antibody and an BDEF14-HRP conjugate. The assay sample and buffer are incubated together with BDEF14-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BDEF14 concentration since BDEF14 from samples and BDEF14-HRP conjugate compete for the anti-BDEF14 antibody binding site. Since the number of sites is limited, as more sites are occupied by BDEF14 from the sample, fewer sites are left to bind BDEF14-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BDEF14 concentration in each sample is interpolated from this standard curve.

109732529

AAI16199.1

7,718 Da

Beta Defensins Pathway (1001262); Defensins Pathway (1001260); Immune System Pathway (971216); Innate Immune System Pathway (971212)

Has antibacterial activity.

48-Strip-Wells

440 USD

96-Strip-Wells

640

5x96-Strip-Wells

2895

10x96-Strip-Wells

5415