catalog number :
MBS747413
products type :
ELISA Kit
products full name :
Canine Tamm Horsfall Protein ELISA Kit
products short name :
Tamm Horsfall Protein
other names :
Tamm-Horsfall protein; Uromodulin; Tamm-Horsfall urinary glycoprotein; THP
other gene names :
Umod; THP
uniprot entry name :
UROM_RAT
specificity :
This assay has high sensitivity and excellent specificity for detection of THP. No significant cross-reactivity or interference between THP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between THP and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.
other info2 :
Intended Uses: This THP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine THP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
products description :
Principle of the assay: THP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-THP antibody and an THP-HRP conjugate. The assay sample and buffer are incubated together with THP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the THP concentration since THP from samples and THP-HRP conjugate compete for the anti-THP antibody binding site. Since the number of sites is limited, as more sites are occupied by THP from the sample, fewer sites are left to bind THP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The THP concentration in each sample is interpolated from this standard curve.
ncbi acc num :
AAB33313.1
ncbi mol weight :
71,062 Da