ELISA/assay

Human Anti Granulocyte macrophage colony stimulating factor antibody ELISA Kit

MBS747325

48-Strip-Wells

470 USD

ELISA Kit

Human Anti Granulocyte macrophage colony stimulating factor antibody ELISA Kit

Anti Granulocyte macrophage colony stimulating factor antibody

GM-CSF; Granulocyte-macrophage colony-stimulating factor; granulocyte-macrophage colony-stimulating factor; CSF; molgramostin; sargramostim; colony stimulating factor 2 (granulocyte-macrophage); Colony-stimulating factor; CSF; Molgramostin; Sargramostim

GM CSF

CSF2; CSF2; GMCSF; GMCSF; GM-CSF; CSF

CSF2_HUMAN

Human

This assay has high sensitivity and excellent specificity for detection of anti-GM-CSF. No significant cross-reactivity or interference between anti-GM-CSF and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between anti-GM-CSF and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/ml.

Intended Uses: This anti-GM-CSF ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human anti-GM-CSF. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Immunology

Principle of the assay: Anti-GM-CSF ELISA kit applies the competitive enzyme immunoassay technique utilizing a GM-CSF antigen and an anti-GM-CSF-HRP conjugate. The assay sample and buffer are incubated together with anti-GM-CSF-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the anti-GM-CSF concentration since anti-GM-CSF from samples and anti-GM-CSF-HRP conjugate compete for the GM-CSF antigen binding site. Since the number of sites is limited, as more sites are occupied by anti-GM-CSF from the sample, fewer sites are left to bind anti-GM-CSF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The anti-GM-CSF concentration in each sample is interpolated from this standard curve.

183364

AAA52578.1

16,295 Da

Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway (137993); Calcium Signaling In The CD4+ TCR Pathway (137941); Cytokine Signaling In Immune System Pathway (366171); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Cytokines And Inflammatory Response Pathway (198794); Fc Epsilon RI Signaling Pathway (83082); Fc Epsilon RI Signaling Pathway (493)

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. [provided by RefSeq, Jul 2008]

GMCSF: Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. Belongs to the GM-CSF family. Protein type: Cytokine; Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 5q31.1. Cellular Component: extracellular space; extracellular region. Molecular Function: protein binding; growth factor activity; granulocyte macrophage colony-stimulating factor receptor binding; cytokine activity. Biological Process: negative regulation of cytolysis; epithelial fluid transport; macrophage activation; embryonic placenta development; positive regulation of tyrosine phosphorylation of Stat5 protein; positive regulation of interleukin-23 production; positive regulation of cell proliferation; immune response; myeloid dendritic cell differentiation; positive regulation of DNA replication

48-Strip-Wells

470 USD

96-Strip-Wells

675