ELISA/assay

Human Vitamin K ELISA Kit

MBS746981

48-Strip-Wells

470 USD

ELISA Kit

Human Vitamin K ELISA Kit

Vitamin K

VK

Human

This assay has high sensitivity and excellent specificity for detection of VK. No significant cross-reactivity or interference between VK and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between VK and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Quantitative Competitive. Sensitivity: 1.0 pg/mL.

Intended Uses: This VK ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human VK. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: VK ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-VK antibody and an VK-HRP conjugate. The assay sample and buffer are incubated together with VK-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the VK concentration since VK from samples and VK-HRP conjugate compete for the anti-VK antibody binding site. Since the number of sites is limited, as more sites are occupied by VK from the sample, fewer sites are left to bind VK-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The VK concentration in each sample is interpolated from this standard curve.

48-Strip-Wells

470 USD

96-Strip-Wells

675