ELISA/assay

Canine Histone ELISA Kit

MBS746638

48-Strip-Wells

440 USD

ELISA Kit

Canine Histone ELISA Kit

[Histone]

[histone; Histone H2A.Z; histone H2A.Z; H2AZ histone; H2A histone family, member Z]

[Histone]

[H2AFZ; H2AFZ; H2AZ; H2A.z; H2A/z; H2A.Z-1; H2AZ; H2A/z]

H2AZ_HUMAN

Canine

This assay has high sensitivity and excellent specificity for detection of HIF-1alpha. No significant cross-reactivity or interference between HIF-1alpha and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between HIF-1alpha and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate. Assay Type: Quantitative Competitive or Sandwich. Sensitivity: 0.1 ng/mL.

Cell Biology

Intended Uses: This HIF-1alpha ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat HIF-1alpha. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: HIF-1alpha ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for HIF-1alpha. Standards or samples are then added to the microtiter plate wells and HIF-1alpha if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of HIF-1alpha present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for HIF-1alpha are added to each well to "sandwich" the HIF-1alpha immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain HIF-1alpha and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HIF-1alpha concentration in each sample is interpolated from this standard curve.

3649600

AAC61625.1

13,553 Da

Alcoholism Pathway (585563); Alcoholism Pathway (587116); Amyloids Pathway (366238); C-MYB Transcription Factor Network Pathway (138073); Cell Cycle Pathway (530733); Cell Cycle, Mitotic Pathway (105765); Cellular Senescence Pathway (905991); Cellular Responses To Stress Pathway (645258); Chromatin Modifying Enzymes Pathway (941403); Chromatin Organization Pathway (941402)

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent member of the histone H2A family that is distinct from other members of the family. Studies in mice have shown that this particular histone is required for embryonic development and indicate that lack of functional histone H2A leads to embryonic lethality. [provided by RefSeq, Jul 2008]

H2AZ: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. H2A or its variant H2AFZ forms an heterodimer with H2B. H2AFZ interacts with INCENP. Belongs to the histone H2A family. Protein type: DNA-binding. Chromosomal Location of Human Ortholog: 4q24. Cellular Component: Barr body; nucleosome; nuclear heterochromatin; nucleus. Molecular Function: protein binding; nucleosomal DNA binding; chromatin DNA binding; protein heterodimerization activity. Biological Process: positive regulation of transcription from RNA polymerase II promoter

48-Strip-Wells

440 USD

96-Strip-Wells

640

5x96-Strip-Wells

2895

10x96-Strip-Wells

5415