product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Bovine Collagenase, Type II ELISA Kit
catalog :
MBS746413
quantity :
48-Strip-Wells
price :
470 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS746413
products type :
ELISA Kit
products full name :
Bovine Collagenase, Type II ELISA Kit
products short name :
Collagenase, Type II
other names :
collagenase, partial; Collagenase 3; collagenase 3; MMP-13; UMRCASE; collagenase-3; matrix metalloproteinase 13; matrix metalloproteinase-13; matrix metallopeptidase 13; Matrix metalloproteinase-13; MMP-13; UMRCASE
products gene name :
Collagenase II
other gene names :
Mmp13; Mmp13; MMP-13
uniprot entry name :
MMP13_RAT
reactivity :
Bovine
specificity :
This assay has high sensitivity and excellent specificity for detection of CoL-2. No significant cross-reactivity or interference between CoL-2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CoL-2 and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.
other info2 :
Intended Uses: This CoL-2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine CoL-2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
products description :
Principle of the assay: CoL-2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CoL-2 antibody and an CoL-2-HRP conjugate. The assay sample and buffer are incubated together with CoL-2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CoL-2 concentration since CoL-2 from samples and CoL-2-HRP conjugate compete for the anti-CoL-2 antibody binding site. Since the number of sites is limited, as more sites are occupied by CoL-2 from the sample, fewer sites are left to bind CoL-2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CoL-2 concentration in each sample is interpolated from this standard curve.
ncbi gi num :
203499
ncbi acc num :
AAA72124.1
ncbi mol weight :
53,375 Da
ncbi pathways :
Activation Of Matrix Metalloproteinases Pathway (1009937); Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway (1009926); Collagen Degradation Pathway (1009938); Collagen Formation Pathway (1009924); Degradation Of The Extracellular Matrix Pathway (1009936); Endochondral Ossification Pathway (219771); Extracellular Matrix Organization Pathway (1009923); Matrix Metalloproteinases Pathway (198492)
ncbi summary :
enzyme that degrades collagen type I [RGD, Feb 2006]
size1 :
48-Strip-Wells
price1 :
470 USD
size2 :
96-Strip-Wells
price2 :
675
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

"MyBioSource offers the best products at unbeatable prices."

Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

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