product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Human Interleukin 36 beta ELISA Kit
catalog :
MBS746285
quantity :
48-Strip-Wells
price :
470 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS746285
products type :
ELISA Kit
products full name :
Human Interleukin 36 beta ELISA Kit
products short name :
Interleukin 36 beta
products name syn :
Human Interleukin 36 b ELISA Kit
other names :
interleukin-36 beta isoform 2; Interleukin-36 beta; interleukin-36 beta; FIL1 eta; IL-1 eta; IL-1F8 (FIL1-eta); interleukin-1 eta; interleukin 1, eta; interleukin-1 homolog 2; Interleukin-1 Superfamily e; family of interleukin 1-eta; interleukin-1 family member 8; IL1F8 (Canonical product IL-1F8a); interleukin 1 family, member 8 (eta); interleukin 36, beta; FIL1 eta; Interleukin-1 eta; IL-1 eta; Interleukin-1 family member 8; IL-1F8; Interleukin-1 homolog 2; IL-1H2
products gene name :
IL 36 beta/ IL 1F8/FIL 1h/IL 1H2
products gene name syn :
IL 36 b/ IL 1F8/FIL 1h/IL 1H2
other gene names :
IL36B; IL36B; FIL1; FIL1H; IL1F8; IL1H2; IL-1F8; IL-1H2; IL1-ETA; FIL1-(ETA); FILI-(ETA); IL1F8; IL1H2; IL-1 eta; IL-1F8; IL-1H2
uniprot entry name :
IL36B_HUMAN
reactivity :
Human
specificity :
This assay has high sensitivity and excellent specificity for detection of IL-36beta. No significant cross-reactivity or interference between IL-36beta and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IL-36beta and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.
other info2 :
Intended Uses: This IL-36beta ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human IL-36beta. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
products categories :
Immunology
products description :
Principle of the Assay: IL-36beta ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IL-36beta antibody and an IL-36beta-HRP conjugate. The assay sample and buffer are incubated together with IL-36beta-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL-36beta concentration since IL-36beta from samples and IL-36beta-HRP conjugate compete for the anti-IL-36beta antibody binding site. Since the number of sites is limited, as more sites are occupied by IL-36beta from the sample, fewer sites are left to bind IL-36beta-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL-36beta concentration in each sample is interpolated from this standard curve.
ncbi gi num :
27894313
ncbi acc num :
NP_775270.1
ncbi gb acc num :
NM_173178.2
ncbi mol weight :
17,702 Da
ncbi summary :
The protein encoded by this gene is a member of the interleukin 1 cytokine family. Protein structure modeling indicated that this cytokine may contain a 12-stranded beta-trefoil structure that is conserved between IL1A (IL-A alpha) and IL1B (IL-1 beta). This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. Two alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
uniprot summary :
IL36B: Stimulates production of interleukin-6 and interleukin-8 in synovial fibrobasts, articular chondrocytes and mature adipocytes. Induces expression of a number of antimicrobial peptides including beta-defensins 4 and 103 as well as a number of matrix metalloproteases. Belongs to the IL-1 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Cytokine. Chromosomal Location of Human Ortholog: 2q14. Cellular Component: extracellular space; extracellular region. Molecular Function: interleukin-1 receptor binding; cytokine activity. Biological Process: positive regulation of T cell differentiation; cytokine and chemokine mediated signaling pathway; positive regulation of interleukin-6 production; innate immune response; immune response; inflammatory response
size1 :
48-Strip-Wells
price1 :
470 USD
size2 :
96-Strip-Wells
price2 :
675
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

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