catalog number :
MBS746167
products type :
ELISA Kit
products full name :
Rabbit Tartrate Resistant Acid Phosphatase+B742 ELISA Kit
products short name :
Tartrate Resistant Acid Phosphatase+B742
other names :
traP; Protein TraP
products gene name :
TRAP
other gene names :
traP; ECOK12F079
uniprot entry name :
TRAP_ECOLI
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.
other info2 :
Intended Uses: This TRACP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit TRACP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
products categories :
Immunology
products description :
Principle of the Assay: TRAP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TRAP antibody and a TRAP-HRP conjugate. The assay sample and buffer are incubated together with TRAP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TRAP concentration since TRAP from samples and TRAP-HRP conjugate compete for the anti-TRAP antibody binding site. Since the number of sites is limited, as more sites are occupied by TRAP from the sample, fewer sites are left to bind TRAP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TRAP concentration in each sample is interpolated from this standard curve.
ncbi acc num :
BAA97949.1
ncbi mol weight :
21,961 Da