ELISA/assay

Rat Programmed cell death 1 ligand 1 ELISA Kit

MBS745856

96 Strip Wells

675 USD

ELISA Kit

Rat Programmed cell death 1 ligand 1 ELISA Kit

Programmed cell death 1 ligand 1

programmed cell death 1 ligand 1 isoform a; Programmed cell death 1 ligand 1; programmed cell death 1 ligand 1; B7 homolog 1; CD274 antigen; PDCD1 ligand 1; programmed death ligand 1; CD274 molecule; B7 homolog 1; B7-H1; CD_antigen: CD274

PDL1/B7-H1/CD274

CD274; CD274; B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1; B7H1; PDCD1L1; PDCD1LG1; PDL1; PD-L1; PDCD1 ligand 1; Programmed death ligand 1; B7-H1

PD1L1_HUMAN

Rat

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Sensitivity: The sensitivity in this assay is 0.1 ng/mL.

Intended Uses: This PDL1/B7-H1/CD274 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat PDL1/B7-H1/CD274. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: PDL1/B7-H1/CD274 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PDL1/B7-H1/CD274 antibody and an PDL1/B7-H1/CD274-HRP conjugate. The assay sample and buffer are incubated together with PDL1/B7-H1/CD274-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PDL1/B7-H1/CD274 concentration since PDL1/B7-H1/CD274 from samples and PDL1/B7-H1/CD274-HRP conjugate compete for the anti-PDL1/B7-H1/CD274 antibody binding site. Since the number of sites is limited, as more sites are occupied by PDL1/B7-H1/CD274 from the sample, fewer sites are left to bind PDL1/B7-H1/CD274-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PDL1/B7-H1/CD274 concentration in each sample is interpolated from this standard curve.

7661534

NP_054862.1

NM_014143.3

20,454 Da

Adaptive Immune System Pathway 366160!!Cell Adhesion Molecules (CAMs) Pathway 83069!!Cell Adhesion Molecules (CAMs) Pathway 480!!Costimulation By The CD28 Family Pathway 119552!!Immune System Pathway 106386!!PD-1 Signaling Pathway 119557

96 Strip Wells

675 USD