Sheep Syndecan-1/CD138 ELISA Kit | MyBioSource MBS745791 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Sheep Syndecan-1/CD138 ELISA Kit

catalog :

MBS745791

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS745791

products type :

ELISA Kit

products full name :

Sheep Syndecan-1/CD138 ELISA Kit

products short name :

Syndecan-1/CD138

other names :

syndecan-1; Syndecan-1; syndecan-1; CD138 antigen; syndecan proteoglycan 1; heparan sulfate proteoglycan fibroblast growth factor receptor; syndecan 1; CD_antigen: CD138

products gene name :

SDC1/CD138

other gene names :

SDC1; SDC1; SDC; CD138; SYND1; syndecan; SDC; SYND1

uniprot entry name :

SDC1_HUMAN

reactivity :

Sheep

specificity :

This assay has high sensitivity and excellent specificity for detection of SDC-1. No significant cross-reactivity or interference between SDC-1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SDC-1 and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 ng/mL.

other info2 :

Intended Uses: This SDC-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep SDC-1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products categories :

Cell Biology

products description :

Principle of the assay: SDC-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SDC-1 antibody and an SDC-1-HRP conjugate. The assay sample and buffer are incubated together with SDC-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SDC-1 concentration since SDC-1 from samples and SDC-1-HRP conjugate compete for the anti-SDC-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by SDC-1 from the sample, fewer sites are left to bind SDC-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SDC-1 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

55749480

ncbi acc num :

NP_001006947.1

ncbi gb acc num :

NM_001006946.1

ncbi mol weight :

32,462 Da

ncbi pathways :

A Tetrasaccharide Linker Sequence Is Required For GAG Synthesis Pathway (645305); Arf6 Trafficking Events Pathway (137954); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Chondroitin Sulfate/dermatan Sulfate Metabolism Pathway (645308); Chylomicron-mediated Lipid Transport Pathway (106157); Disease Pathway (530764); Diseases Associated With Visual Transduction Pathway (771581); ECM-receptor Interaction Pathway (83068); ECM-receptor Interaction Pathway (479)

ncbi summary :

The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding, cell signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan-1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. Altered syndecan-1 expression has been detected in several different tumor types. While several transcript variants may exist for this gene, the full-length natures of only two have been described to date. These two represent the major variants of this gene and encode the same protein. [provided by RefSeq, Jul 2008]

uniprot summary :

syndecan-1: Cell surface proteoglycan that bears both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix. Belongs to the syndecan proteoglycan family. Protein type: Membrane protein, integral; Extracellular matrix; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 2p24.1. Cellular Component: lysosomal lumen; cell surface; protein complex; focal adhesion; Golgi lumen; integral to plasma membrane; cytoplasm; plasma membrane; external side of plasma membrane. Molecular Function: protein C-terminus binding; protein binding; glycoprotein binding. Biological Process: phototransduction, visible light; extracellular matrix organization and biogenesis; response to cAMP; myoblast development; wound healing; glycosaminoglycan metabolic process; striated muscle cell development; response to toxin; response to glucocorticoid stimulus; lipoprotein metabolic process; pathogenesis; Wnt receptor signaling pathway through beta-catenin; Sertoli cell development; chondroitin sulfate metabolic process; odontogenesis; glycosaminoglycan biosynthetic process; glycosaminoglycan catabolic process; response to hydrogen peroxide; ureteric bud development; carbohydrate metabolic process; retinoid metabolic process; response to calcium ion; inflammatory response

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage