ELISA/assay

Human Integrin beta1 ELISA Kit

MBS745332

48-Strip-Wells

470 USD

ELISA Kit

Human Integrin beta1 ELISA Kit

Integrin beta1

Human Integrin b1 ELISA Kit

integrin beta-1 isoform 1A; Integrin beta-1; integrin beta-1; glycoprotein IIa; integrin VLA-4 beta subunit; very late activation protein, beta polypeptide; integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12); Fibronectin receptor subunit beta; Glycoprotein IIa; GPIIA; VLA-4 subunit beta; CD_antigen: CD29

ITGbeta1

ITGb1

ITGB1; ITGB1; CD29; FNRB; MDF2; VLAB; GPIIA; MSK12; VLA-BETA; FNRB; MDF2; MSK12; GPIIA

ITB1_HUMAN

Human

This assay has high sensitivity and excellent specificity for detection of ITGbeta1. No significant cross-reactivity or interference between ITGbeta1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ITGbeta1 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Sensitivity: 0.1 ng/mL. Assay Type: Competitive

Intended Uses: This ITGbeta1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ITGbeta1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Signal Transduction

Principle of the Assay: ITGbeta1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ITGbeta1 antibody and an ITGbeta1-HRP conjugate. The assay sample and buffer are incubated together with ITGbeta1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ITGbeta1 concentration since ITGbeta1 from samples and ITGbeta1-HRP conjugate compete for the anti-ITGbeta1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ITGbeta1 from the sample, fewer sites are left to bind ITGbeta1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ITGbeta1 concentration in each sample is interpolated from this standard curve.

19743823

NP_596867.1

NM_133376.2

88,884 Da

Adaptive Immune System Pathway (366160); Angiopoietin Receptor Tie2-mediated Signaling Pathway (137917); Arf6 Trafficking Events Pathway (137954); Arrhythmogenic Right Ventricular Cardiomyopathy Pathway (672454); Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway (117293); Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway (116129); Axon Guidance Pathway (83065); Axon Guidance Pathway (476); Axon Guidance Pathway (105688); Bacterial Invasion Of Epithelial Cells Pathway (149807)

Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

ITGB1: an integral membrane protein that heterodimerizes with an alpha-3 chain, forming a receptor for many extracellular-matrix proteins including fibronectin, laminin, collagen, epiligrin and thrombospondin. Beta 1 integrins recognize the amino-acid motif RGD in a wide array of ligands. Five alternatively spliced variants with alternate carboxy termini have been described. Two alternatively spliced isoforms have been described. Isoform beta-1a is widely expressed; other isoforms are generally expressed with a more restricted distribution. Isoform beta-1b is expressed in skin, liver, skeletal muscle, cardiac muscle, placenta, umbilical vein endothelial cells, neuroblastoma cells, lymphoma cells, hepatoma cells and astrocytoma cells. Isoforms beta-1c and beta-1c-2 are expressed in muscle, kidney, liver, placenta, cervical epithelium, umbilical vein endothelial cells, fibroblast cells, embryonal kidney cells, platelets and several blood cell lines. Isoform beta-c-2, rather than isoform beta-1c, is selectively expressed in primary t-cells. Isoform beta-1c is expressed in nonproliferating and differentiated prostate gland epithelial cells. Isoform beta-1d is expressed specifically in striated muscle (skeletal and cardiac muscle). Protein type: Cell surface; Cell adhesion; Motility/polarity/chemotaxis; Membrane protein, integral; Receptor, misc. Chromosomal Location of Human Ortholog: 10p11.2. Cellular Component: focal adhesion; cell surface; dendritic spine; acrosome; lipid raft; ruffle; recycling endosome; hemidesmosome; membrane; lamellipodium; cytoplasm; plasma membrane; melanosome; basement membrane; integrin complex; neuromuscular junction; receptor complex; sarcolemma; cleavage furrow; filopodium; external side of plasma membrane. Molecular Function: protein domain specific binding; protease binding; metal ion binding; laminin binding; alpha-actinin binding; actin binding; protein kinase binding; peptide binding; viral receptor activity; integrin binding; protein binding; protein heterodimerization activity; fibronectin binding; cell adhesion molecule binding; glycoprotein binding. Biological Process: axon guidance; entry of virus into host cell; extracellular matrix organization and biogenesis; maternal process involved in pregnancy; regulation of cell cycle; axon extension; positive regulation of apoptosis; cell-matrix adhesion; mesodermal cell differentiation; cell fate specification; positive regulation of endocytosis; cardiac muscle cell differentiation; negative regulation of cell proliferation; leukocyte adhesion; positive regulation of MAPKKK cascade; transforming growth factor beta receptor signaling pathway; germ cell migration; positive regulation of cell proliferation; tissue homeostasis; visual learning; negative regulation of cell projection organization and biogenesis; integrin-mediated signaling pathway; response to drug; cell migration; regulation of immune response; in utero embryonic development; dendrite morphogenesis; sarcomere organization; protein transport within lipid bilayer; cell-substrate adhesion; cellular calcium ion homeostasis; formation of radial glial scaffolds; heterotypic cell-cell adhesion; negative regulation of neuron differentiation; positive regulation of peptidyl-tyrosine phosphorylation; cell migration during sprouting angiogenesis; negative regulation of Rho protein signal transduction; B cell differentiation; cell-cell adhesion mediated by integrin; stress fiber formation; calcium-independent cell-matrix adhesion; cellular defense response; response to activity; homophilic cell adhesion; leukocyte tethering or rolling; blood coagulation; leukocyte migration; regulation of G-protein coupled receptor protein signaling pathway; positive regulation of cell migration; G1/S transition of mitotic cell cycle

48-Strip-Wells

470 USD

96-Strip-Wells

675