ELISA/assay

Human Soluble Cytotoxic T Lymphocyte Associated Antigen 4 ELISA Kit

MBS745301

48-Strip-Wells

470 USD

ELISA Kit

Human Soluble Cytotoxic T Lymphocyte Associated Antigen 4 ELISA Kit

Soluble Cytotoxic T Lymphocyte Associated Antigen 4

sCTLA4, partial; SCTLA4; cytotoxic T-lymphocyte protein 4; soluble form; CD152 antigen; transmembrane form; cytotoxic T-lymphocyte-associated protein 4; SCTLA4

sCTLA4

Ctla4; CTLA-4; Cd152; CTLA-4; sCTLA4

Q792X1_RAT

Human

This assay has high sensitivity and excellent specificity for detection of sCTLA4. No significant cross-reactivity or interference between sCTLA4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between sCTLA4 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This sCTLA4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human sCTLA4. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Signal Transduction

Principle of the assay: sCTLA4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-sCTLA4 antibody and an sCTLA4-HRP conjugate. The assay sample and buffer are incubated together with sCTLA4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the sCTLA4 concentration since sCTLA4 from samples and sCTLA4-HRP conjugate compete for the anti-sCTLA4 antibody binding site. Since the number of sites is limited, as more sites are occupied by sCTLA4 from the sample, fewer sites are left to bind sCTLA4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The sCTLA4 concentration in each sample is interpolated from this standard curve.

4154201

AAD04806.1

2,501 Da

Adaptive Immune System Pathway (1010925); Autoimmune Thyroid Disease Pathway (83509); Autoimmune Thyroid Disease Pathway (533); CTLA4 Inhibitory Signaling Pathway (1010935); Cell Adhesion Molecules (CAMs) Pathway (83461); Cell Adhesion Molecules (CAMs) Pathway (480); Costimulation By The CD28 Family Pathway (1010931); Immune System Pathway (1010924); Rheumatoid Arthritis Pathway (200306); Rheumatoid Arthritis Pathway (200269)

immunoregulatory receptor expressed on the surface of T and B lymphocytes [RGD, Feb 2006]

48-Strip-Wells

470 USD

96-Strip-Wells

675