Monkey Fibroblast Growth Factor 19 ELISA Kit | MyBioSource MBS745173 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Monkey Fibroblast Growth Factor 19 ELISA Kit

catalog :

MBS745173

quantity :

48-Strip-Wells

price :

440 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS745173

products type :

ELISA Kit

products full name :

Monkey Fibroblast Growth Factor 19 ELISA Kit

products short name :

[Fibroblast Growth Factor 19]

other names :

[fibroblast growth factor 19; Fibroblast growth factor 19; fibroblast growth factor 19; FGF-19; fibroblast growth factor 19]

products gene name :

[FGF19]

other gene names :

[FGF19; FGF19; UNQ334/PRO533; FGF-19]

uniprot entry name :

FGF19_HUMAN

reactivity :

Monkey

specificity :

This assay has high sensitivity and excellent specificity for detection of FGF-19. No significant cross-reactivity or interference between FGF-19 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between FGF-19 and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate. Assay Type: Quantitative Competitive. Sensitivity: 1.0 pg/mL.

products categories :

Signal Transduction

products description :

Intended Uses: This FGF-19 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine FGF-19. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: FGF-19 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-FGF-19 antibody and an FGF-19-HRP conjugate. The assay sample and buffer are incubated together with FGF-19-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FGF-19 concentration since FGF-19 from samples and FGF-19-HRP conjugate compete for the anti-FGF-19 antibody binding site. Since the number of sites is limited, as more sites are occupied by FGF-19 from the sample, fewer sites are left to bind FGF-19-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FGF-19 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

4826726

ncbi acc num :

NP_005108.1

ncbi gb acc num :

NM_005117.2

ncbi mol weight :

24,003 Da

ncbi pathways :

Adaptive Immune System Pathway (366160); Constitutive PI3K/AKT Signaling In Cancer Pathway (685535); DAP12 Interactions Pathway (685549); DAP12 Signaling Pathway (685550); Disease Pathway (530764); Downstream Signal Transduction Pathway (106385); Downstream Signaling Events Of B Cell Receptor (BCR) Pathway (576250); Downstream Signaling Of Activated FGFR Pathway (160957); FGF Signaling Pathway (137989); FGFR Ligand Binding And Activation Pathway (106344)

ncbi summary :

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes including embryonic development cell growth, morphogenesis, tissue repair, tumor growth and invasion. This growth factor is a high affinity, heparin dependent ligand for FGFR4. Expression of this gene was detected only in fetal but not adult brain tissue. Synergistic interaction of the chick homolog and Wnt-8c has been shown to be required for initiation of inner ear development. [provided by RefSeq, Jul 2008]

uniprot summary :

FGF19: Involved in the suppression of bile acid biosynthesis through down-regulation of CYP7A1 expression, following positive regulation of the JNK and ERK1/2 cascades. Stimulates glucose uptake in adipocytes. Activity requires the presence of KLB and FGFR4. Belongs to the heparin-binding growth factors family. Protein type: Cytokine; Secreted; Cell development/differentiation; Motility/polarity/chemotaxis; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 11q13.1. Cellular Component: extracellular region. Molecular Function: protein binding; growth factor activity; fibroblast growth factor receptor binding. Biological Process: epidermal growth factor receptor signaling pathway; positive regulation of glucose import; nervous system development; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; heart development; positive regulation of cell proliferation; insulin receptor signaling pathway; innate immune response; positive regulation of JNK cascade; positive regulation of protein amino acid phosphorylation; neural crest cell migration

size1 :

48-Strip-Wells

price1 :

440 USD

size2 :

96-Strip-Wells

price2 :

640

size3 :

5x96-Strip-Wells

price3 :

2895

size4 :

10x96-Strip-Wells

price4 :

5415

more info or order :

MyBioSource product webpage