catalog number :
MBS744945
products type :
ELISA Kit
products full name :
Rabbit Immunoglobulin G4 ELISA Kit
products short name :
Immunoglobulin G4
other names :
immunoglobulin G4 heavy chain variable region, partial
products gene name :
IgG4
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich
other info2 :
Intended Uses: This IgG4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit IgG4. This ELISA kit for research use only, not for therapeutic or diagnostic applications. !Intended Uses: This IgG4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit IgG4. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
products categories :
Immunology
products description :
Principle of the Assay: IgG4 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for IgG4. Standards or samples are then added to the microtiter plate wells and IgG4 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of IgG4 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for IgG4 are added to each well to "sandwich" the IgG4 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IgG4 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IgG4 concentration in each sample is interpolated from this standard curve.
ncbi acc num :
AFI81247.1