ELISA/assay

Mouse Neurokinin B ELISA Kit

MBS744693

48-Strip-Wells

470 USD

ELISA Kit

Mouse Neurokinin B ELISA Kit

Neurokinin B

neurokinin B; Tachykinin-3; tachykinin-3; neurokinin b; neuromedin K; gamma tachykinin 3; preprotachykinin B; preprotachykinin-B; tachykinin 3; ZNEUROK1Cleaved into the following chain:Neurokinin-B; NKBAlternative name(s):Neuromedin-K

NKB

TAC3; TAC3; NKB; HH10; NKNB; PRO1155; ZNEUROK1; NKNB; UNQ585/PRO1155; NKB

TKNK_HUMAN

Mouse

This assay has high sensitivity and excellent specificity for detection of NKB. No significant cross-reactivity or interference between NKB and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between NKB and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This NKB ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse NKB. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Neurobiology

Principle of the assay: NKB ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-NKB antibody and an NKB-HRP conjugate. The assay sample and buffer are incubated together with NKB-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NKB concentration since NKB from samples and NKB-HRP conjugate compete for the anti-NKB antibody binding site. Since the number of sites is limited, as more sites are occupied by NKB from the sample, fewer sites are left to bind NKB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NKB concentration in each sample is interpolated from this standard curve.

8572053

AAF76980.1

11,358 Da

Class A/1 (Rhodopsin-like Receptors) Pathway (106357); G Alpha (q) Signalling Events Pathway (106043); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); Gastrin-CREB Signalling Pathway Via PKC And MAPK (645295); Peptide Ligand-binding Receptors Pathway (106358); Signal Transduction Pathway (477114); Signaling By GPCR Pathway (106356); Tachykinin Receptors Bind Tachykinins Pathway (106360)

This gene encodes a member of the tachykinin family of secreted neuropeptides. The encoded protein is primarily expressed in the central and peripheral nervous system and functions as a neurotransmitter. This protein is the ligand for the neurokinin-3 receptor. This protein is also expressed in the outer syncytiotrophoblast of the placenta and may be associated with pregnancy-induced hypertension and pre-eclampsia. Mutations in this gene are associated with normosmic hypogonadotropic hypogonadism. Alternate splicing results in multiple transcript variants. [provided by RefSeq, May 2010]

TAC3: Tachykinins are active peptides which excite neurons, evoke behavioral responses, are potent vasodilators and secretagogues, and contract (directly or indirectly) many smooth muscles. Belongs to the tachykinin family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 12q13.3. Cellular Component: extracellular space; extracellular region. Molecular Function: protein binding; receptor binding. Biological Process: tachykinin signaling pathway; neuropeptide signaling pathway; female pregnancy. Disease: Hypogonadotropic Hypogonadism 10 With Or Without Anosmia

48-Strip-Wells

470 USD

96-Strip-Wells

675