ELISA/assay

Monkey Carboxypeptidase A3 (mast cell) ELISA Kit

MBS744658

48-Strip-Wells

470 USD

ELISA Kit

Monkey Carboxypeptidase A3 (mast cell) ELISA Kit

Carboxypeptidase A3 (mast cell)

carboxypeptidase A3 (mast cell); Mast cell carboxypeptidase A; mast cell carboxypeptidase A; tissue carboxypeptidase A; carboxypeptidase A3 (mast cell); Carboxypeptidase A3

CPA3

CPA3; CPA3; MC-CPA; MC-CPA

CBPA3_HUMAN

Monkey

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive

Intended Uses: This CPA3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey CPA3. This ELISA kit for research use only, not for therapeutic or diagnostic applications. !Intended Uses: This CPA3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey CPA3. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Cell Biology

Principle of the Assay: CPA3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CPA3 antibody and an CPA3-HRP conjugate. The assay sample and buffer are incubated together with CPA3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CPA3 concentration since CPA3 from samples and CPA3-HRP conjugate compete for the anti-CPA3 antibody binding site. Since the number of sites is limited, as more sites are occupied by CPA3 from the sample, fewer sites are left to bind CPA3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CPA3 concentration in each sample is interpolated from this standard curve.

119599308

EAW78902.1

48,670 Da

Metabolism Of Angiotensinogen To Angiotensins Pathway (685555); Metabolism Of Proteins Pathway (106230); Pancreatic Secretion Pathway (169306); Pancreatic Secretion Pathway (169295); Peptide Hormone Metabolism Pathway (771603); Protein Digestion And Absorption Pathway (172847); Protein Digestion And Absorption Pathway (171868); Renin-angiotensin System Pathway (83075); Renin-angiotensin System Pathway (486)

Three different forms of human pancreatic procarboxypeptidase A have been isolated. This gene encodes a form which is obtained as a binary complex of a procarboxypeptidase A with proproteinase E and functions as a secretory granule metalloexopeptidase. [provided by RefSeq, Jan 2009]

CPA3: Three different forms of human pancreatic procarboxypeptidase A have been isolated. This gene encodes a form which is obtained as a binary complex of a procarboxypeptidase A with proproteinase E and functions as a secretory granule metalloexopeptidase. [provided by RefSeq, Jan 2009]. Protein type: EC 3.4.17.1; Protease. Chromosomal Location of Human Ortholog: 3q24. Cellular Component: transport vesicle; extracellular region; secretory granule. Molecular Function: zinc ion binding; metallocarboxypeptidase activity. Biological Process: cellular protein metabolic process; proteolysis; angiotensin maturation

48-Strip-Wells

470 USD

96-Strip-Wells

675