ELISA/assay

Monkey Insulin like Growth Factor 2, Antisense ELISA Kit

MBS744459

48-Strip-Wells

470 USD

ELISA Kit

Monkey Insulin like Growth Factor 2, Antisense ELISA Kit

Insulin like Growth Factor 2, Antisense

IGF2AS protein; Putative insulin-like growth factor 2 antisense gene protein; IGF2 antisense RNA; IGF2 antisense RNA 1; IGF2 antisense gene protein 1; PEG8/IGF2AS protein; Putative insulin-like growth factor 2 antisense gene protein 1; IGF2-AS1

IGF2AS

IGF2-AS; IGF2-AS; PEG8; IGF2AS; IGF2-AS1; IGF2-AS1; IGF2AS; IGF2-AS1

IG2AS_HUMAN

Monkey

This assay has high sensitivity and excellent specificity for detection of IGF2AS. No significant cross-reactivity or interference between IGF2AS and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IGF2AS and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Cancer

Intended Uses: This IGF2AS ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey IGF2AS. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay IGF2AS ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IGF2AS antibody and an IGF2AS-HRP conjugate. The assay sample and buffer are incubated together with IGF2AS-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IGF2AS concentration since IGF2AS from samples and IGF2AS-HRP conjugate compete for the anti-IGF2AS antibody binding site. Since the number of sites is limited, as more sites are occupied by IGF2AS from the sample, fewer sites are left to bind IGF2AS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IGF2AS concentration in each sample is interpolated from this standard curve.

223460516

AAI36586.1

18,035 Da

This gene expresses a paternally imprinted antisense transcript of the insulin-like growth factor 2 gene. The transcript is overexpressed in Wilms' tumor. This gene is predicted to be non-coding because the predicted protein is not conserved in any other species and the majority of transcripts would be candidates for non-sense mediated decay (NMD) if a protein were expressed. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2009]

IGF2AS: . Chromosomal Location of Human Ortholog: 11p15.5

48-Strip-Wells

470 USD

96-Strip-Wells

675