Human Nuclear factor erythroid 2 related factor 2 ELISA Kit | MyBioSource MBS744368 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Nuclear factor erythroid 2 related factor 2 ELISA Kit

catalog :

MBS744368

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS744368

products type :

ELISA Kit

products full name :

Human Nuclear factor erythroid 2 related factor 2 ELISA Kit

products short name :

Nuclear factor erythroid 2 related factor 2

other names :

nuclear factor erythroid 2-related factor 2 isoform 2; Nuclear factor erythroid 2-related factor 2; nuclear factor erythroid 2-related factor 2; HEBP1; NFE2-related factor 2; NF-E2-related factor 2; nuclear factor erythroid-derived 2-like 2; nuclear factor, erythroid derived 2, like 2; nuclear factor, erythroid 2-like 2; HEBP1; Nuclear factor, erythroid derived 2, like 2

products gene name :

Nrf2

other gene names :

NFE2L2; NFE2L2; NRF2; NRF2; NF-E2-related factor 2; NFE2-related factor 2

uniprot entry name :

NF2L2_HUMAN

reactivity :

Human

specificity :

This assay has high sensitivity and excellent specificity for detection of NFE2R2. No significant cross-reactivity or interference between NFE2R2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between NFE2R2 and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

other info2 :

Intended Uses: This NFE2R2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human NFE2R2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products description :

Principle of the assay: NFE2R2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-NFE2R2 antibody and an NFE2R2-HRP conjugate. The assay sample and buffer are incubated together with NFE2R2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NFE2R2 concentration since NFE2R2 from samples and NFE2R2-HRP conjugate compete for the anti-NFE2R2 antibody binding site. Since the number of sites is limited, as more sites are occupied by NFE2R2 from the sample, fewer sites are left to bind NFE2R2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NFE2R2 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

224028257

ncbi acc num :

NP_001138884.1

ncbi gb acc num :

NM_001145412.2

ncbi mol weight :

65,356 Da

ncbi pathways :

AhR Pathway (755436); Keap1-Nrf2 Pathway (198777); Oxidative Stress Pathway (198916); Protein Processing In Endoplasmic Reticulum Pathway (167325); Protein Processing In Endoplasmic Reticulum Pathway (167190); Selenium Metabolism And Selenoproteins Pathway (198846)

ncbi summary :

This gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

uniprot summary :

NRF2: a transcription factor that regulates basal expression and antioxidant induction of a transcription factor for NAD(P)H:quinone oxidoreductase-1 (NQO1) and other detoxifying genes. Targeted for proteasomal degradation by INrf2. Oxidative stress causes its phosphorylation and release from INrf2. Protein type: Transcription factor; DNA-binding. Chromosomal Location of Human Ortholog: 2q31. Cellular Component: nucleoplasm; centrosome; cytoplasm; plasma membrane; chromatin; cytosol; nucleus. Molecular Function: protein domain specific binding; protein binding; DNA binding; transcription factor activity. Biological Process: transcription from RNA polymerase II promoter; proteasomal ubiquitin-dependent protein catabolic process; positive regulation of blood coagulation; unfolded protein response; regulation of embryonic development; proteasomal ubiquitin-independent protein catabolic process; protein ubiquitination; positive regulation of transcription from RNA polymerase II promoter

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage