catalog number :
MBS744339
products type :
ELISA Kit
products full name :
Rat Prothrombin Fragment 1+2 (F1+2) ELISA Kit
products short name :
[Prothrombin Fragment 1+2 (F1+2)]
products gene name :
[DOC]
specificity :
This assay has high sensitivity and excellent specificity for detection of PF1+2. No significant cross-reactivity or interference between PF1+2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PF1+2 and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.
products description :
Intended Uses: This PF1+2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat PF1+2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: PF1+2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PF1+2 antibody and an PF1+2-HRP conjugate. The assay sample and buffer are incubated together with PF1+2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PF1+2 concentration since PF1+2 from samples and PF1+2-HRP conjugate compete for the anti-PF1+2 antibody binding site. Since the number of sites is limited, as more sites are occupied by PF1+2 from the sample, fewer sites are left to bind PF1+2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PF1+2 concentration in each sample is interpolated from this standard curve.
size4 :
10x96-Strip-Wells