product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Rat Mammalian target of rapamycin ELISA Kit
catalog :
MBS744326
quantity :
48-Strip-Wells
price :
470 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS744326
products type :
ELISA Kit
products full name :
Rat Mammalian target of rapamycin ELISA Kit
products short name :
Mammalian target of rapamycin
other names :
mammalian target of rapamycin; Mammalian target of rapamycin; mechanistic target of rapamycin (serine/threonine kinase); mechanistic target of rapamycin (serine/threonine kinase); mammalian target of rapamycin; Mammalian target of rapamycin
products gene name :
mTOR
other gene names :
MTOR; mTOR
uniprot entry name :
D9I5T4_CAPHI
reactivity :
Rat
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL
other info2 :
Intended Uses: This mTOR ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat mTOR. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
products description :
Principle of the Assay: mTOR ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-mTOR antibody and an mTOR-HRP conjugate. The assay sample and buffer are incubated together with mTOR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the mTOR concentration since mTOR from samples and mTOR-HRP conjugate compete for the anti-mTOR antibody binding site. Since the number of sites is limited, as more sites are occupied by mTOR from the sample, fewer sites are left to bind mTOR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The mTOR concentration in each sample is interpolated from this standard curve.
ncbi gi num :
297613711
ncbi acc num :
ADI48287.1
ncbi mol weight :
288,647 Da
ncbi pathways :
AMPK Signaling Pathway (990729); AMPK Signaling Pathway (992181); Acute Myeloid Leukemia Pathway (916173); Acute Myeloid Leukemia Pathway (529); Adipocytokine Signaling Pathway (916110); Adipocytokine Signaling Pathway (505); ErbB Signaling Pathway (916024); ErbB Signaling Pathway (458); Glioma Pathway (916166); Glioma Pathway (522)
size1 :
48-Strip-Wells
price1 :
470 USD
size2 :
96-Strip-Wells
price2 :
675
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

"MyBioSource offers the best products at unbeatable prices."

Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

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