MyBioSource

ELISA/assay

Human Utrophin ELISA Kit

MBS744325

96 Strip Wells

675 USD

MBS744325

ELISA Kit

Human Utrophin ELISA Kit

Utrophin

utrophin; Utrophin; utrophin; DRP-1; dystrophin-related protein 1; utrophin; Dystrophin-related protein 1; DRP-1

UTRN

UTRN; UTRN; DRP; DMDL; DRP1; DMDL; DRP1; DRP-1

UTRO_HUMAN

Human

This assay has high sensitivity and excellent specificity for detection of UTRN. No significant cross-reactivity or interference between UTRN and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between UTRN and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Intended Uses: This UTRN ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human UTRN. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Sensitivity: The sensitivity in this assay is 0.1 ng/mL.

Principle of the assay: UTRN ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-UTRN antibody and an UTRN-HRP conjugate. The assay sample and buffer are incubated together with UTRN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the UTRN concentration since UTRN from samples and UTRN-HRP conjugate compete for the anti-UTRN antibody binding site. Since the number of sites is limited, as more sites are occupied by UTRN from the sample, fewer sites are left to bind UTRN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The UTRN concentration in each sample is interpolated from this standard curve.

110611228

NP_009055.2

NM_007124.2

155,026 Da

This gene shares both structural and functional similarities with the dystrophin gene. It contains an actin-binding N-terminus, a triple coiled-coil repeat central region, and a C-terminus that consists of protein-protein interaction motifs which interact with dystroglycan protein components. The protein encoded by this gene is located at the neuromuscular synapse and myotendinous junctions, where it participates in post-synaptic membrane maintenance and acetylcholine receptor clustering. Mouse studies suggest that this gene may serve as a functional substitute for the dystrophin gene and therefore, may serve as a potential therapeutic alternative to muscular dystrophy which is caused by mutations in the dystrophin gene. Alternative splicing of the utrophin gene has been described; however, the full-length nature of these variants has not yet been determined. [provided by RefSeq, Jul 2008]

96 Strip Wells

675 USD