ELISA/assay

Sheep Calcitriol ELISA Kit (1,25OH-VD3) ELISA Kit

MBS744322

96 Strip Wells

675 USD

ELISA Kit

Sheep Calcitriol ELISA Kit (1,25OH-VD3) ELISA Kit

Calcitriol ELISA Kit (1,25OH-VD3)

1,25OH-VD3

Sheep

Store all reagents at 2-8 degree C

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Intended Uses: This 1,25OH-VD3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep 1,25OH-VD3. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Sensitivity: The sensitivity in this assay is 1.0 pg/mL.

Principle of the Assay: 1,25OH-VD3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-1,25OH-VD3 antibody and an 1,25OH-VD3-HRP conjugate. The assay sample and buffer are incubated together with 1,25OH-VD3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the 1,25OH-VD3 concentration since 1,25OH-VD3 from samples and 1,25OH-VD3-HRP conjugate compete for the anti-1,25OH-VD3 antibody binding site. Since the number of sites is limited, as more sites are occupied by 1,25OH-VD3 from the sample, fewer sites are left to bind 1,25OH-VD3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The 1,25OH-VD3 concentration in each sample is interpolated from this standard curve.

96 Strip Wells

675 USD