ELISA/assay

Human Phosphorylated c Jun N terminal kinases/stress activated protein kinase ELISA Kit

MBS744311

48-Strip-Wells

470 USD

ELISA Kit

Human Phosphorylated c Jun N terminal kinases/stress activated protein kinase ELISA Kit

Phosphorylated c Jun N terminal kinases/stress activated protein kinase

P JNK/SAPK

Human

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

Intended Uses: This P-JNK ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human P-JNK. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: P-JNK ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-P-JNK antibody and an P-JNK-HRP conjugate. The assay sample and buffer are incubated together with P-JNK-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the P-JNK concentration since P-JNK from samples and P-JNK-HRP conjugate compete for the anti-P-JNK antibody binding site. Since the number of sites is limited, as more sites are occupied by P-JNK from the sample, fewer sites are left to bind P-JNK-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The P-JNK concentration in each sample is interpolated from this standard curve.

48-Strip-Wells

470 USD

96-Strip-Wells

675