ELISA/assay

Human Transcription factor Maf (MAF) ELISA Kit

MBS744278

48-Strip-Wells

470 USD

ELISA Kit

Human Transcription factor Maf (MAF) ELISA Kit

Transcription factor Maf (MAF)

transcription factor Maf isoform b; Transcription factor Maf; transcription factor Maf; c-maf proto-oncogene; proto-oncogene c-Maf; T lymphocyte c-maf long form; Avian musculoaponeurotic fibrosarcoma (MAF) protooncogene; v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog; Proto-oncogene c-Maf; V-maf musculoaponeurotic fibrosarcoma oncogene homolog

MAF

MAF; MAF; CCA4; c-MAF

MAF_HUMAN

Human

This assay has high sensitivity and excellent specificity for detection of MAF. No significant cross-reactivity or interference between MAF and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MAF and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 mug/mL.

Intended Uses: This MAF ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human MAF. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Epigenetics and Nuclear Signaling

. Principle of the assay: MAF ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MAF antibody and an MAF-HRP conjugate. The assay sample and buffer are incubated together with MAF-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MAF concentration since MAF from samples and MAF-HRP conjugate compete for the anti-MAF antibody binding site. Since the number of sites is limited, as more sites are occupied by MAF from the sample, fewer sites are left to bind MAF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MAF concentration in each sample is interpolated from this standard curve.

73427806

NP_001026974.1

NM_001031804.2

41,961 Da

C-MYB Transcription Factor Network Pathway (138073); Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway (137993); Inflammatory Bowel Disease (IBD) Pathway (842771); Inflammatory Bowel Disease (IBD) Pathway (842797); Keap1-Nrf2 Pathway (198777); Transcriptional Misregulation In Cancer Pathway (523016); Transcriptional Misregulation In Cancer Pathway (522987)

The protein encoded by this gene is a DNA-binding, leucine zipper-containing transcription factor that acts as a homodimer or as a heterodimer. Depending on the binding site and binding partner, the encoded protein can be a transcriptional activator or repressor. This protein plays a role in the regulation of several cellular processes, including embryonic lens fiber cell development, increased T-cell susceptibility to apoptosis, and chondrocyte terminal differentiation. Defects in this gene are a cause of juvenile-onset pulverulent cataract as well as congenital cerulean cataract 4 (CCA4). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2010]

MAF: Acts as a transcriptional activator or repressor. Involved in embryonic lens fiber cell development. Recruits the transcriptional coactivators CREBBP and/or EP300 to crystallin promoters leading to up-regulation of crystallin gene during lens fiber cell differentiation. Activates the expression of IL4 in T helper 2 (Th2) cells. Increases T-cell susceptibility to apoptosis by interacting with MYB and decreasing BCL2 expression. Together with PAX6, transactivates strongly the glucagon gene promoter through the G1 element. Activates transcription of the CD13 proximal promoter in endothelial cells. Represses transcription of the CD13 promoter in early stages of myelopoiesis by affecting the ETS1 and MYB cooperative interaction. Involved in the initial chondrocyte terminal differentiation and the disappearance of hypertrophic chondrocytes during endochondral bone development. Binds to the sequence 5 -[GT]G[GC]N[GT]NCTCAGNN-3 in the L7 promoter. Binds to the T-MARE (Maf response element) sites of lens-specific alpha- and beta-crystallin gene promoters. Binds element G1 on the glucagon promoter. Binds an AT-rich region adjacent to the TGC motif (atypical Maf response element) in the CD13 proximal promoter in endothelial cells. When overexpressed, represses anti-oxidant response element (ARE)- mediated transcription. Involved either as an oncogene or as a tumor suppressor, depending on the cell context. Binds to the ARE sites of detoxifying enzyme gene promoters. Homodimer or heterodimer with other bHLH-Zip transcription factors. Binds DNA as a homodimer or as a heterodimer. Heterotetramer of two MAF and two USF2. Interacts with PAX6; the interaction is direct. Interacts with MYB; interaction takes place weakly in normal T-cells and increases in T-cells following stimulation through the TCR engagement. Interacts with MYB; the ternary complex formed with MYB and the CD13 promoter is regulated in response to differentiating signals. Interacts with USF2; the interaction inhibits its DNA-binding activity on the L7 promoter. Interacts with CREBBP, EP300 and ETS1. Up-regulated with tert-butyl hydroquinone (t-BHQ). Expressed in endothelial cells. Belongs to the bZIP family. Maf subfamily. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Oncoprotein; Cell development/differentiation; Tumor suppressor; DNA-binding; Transcription factor. Chromosomal Location of Human Ortholog: 16q22-q23. Cellular Component: cytoplasm; chromatin; nucleus. Molecular Function: sequence-specific DNA binding. Biological Process: transcription from RNA polymerase II promoter; cytokine production; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; regulation of chondrocyte differentiation; cell development; inner ear development. Disease: Cataract 21, Multiple Types; Ayme-gripp Syndrome

48-Strip-Wells

470 USD

96-Strip-Wells

675