ELISA/assay

Human UL16 Binding protein 1 ELISA Kit

MBS744273

48-Strip-Wells

470 USD

ELISA Kit

Human UL16 Binding protein 1 ELISA Kit

UL16 Binding protein 1

UL16 binding protein 1; NKG2D ligand 1; NKG2D ligand 1; N2DL-1; NKG2DL1; alcan-beta; UL16-binding protein 1; retinoic acid early transcript 1I; UL16 binding protein 1; ALCAN-beta; Retinoic acid early transcript 1I; UL16-binding protein 1

ULBP1

ULBP1; ULBP1; RAET1I; N2DL1; RAET1I; N2DL-1; NKG2DL1

N2DL1_HUMAN

Human

This assay has high sensitivity and excellent specificity for detection of ULBP1. No significant cross-reactivity or interference between ULBP1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ULBP1 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This ULBP1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ULBP1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Immunology

Principle of the assay: ULBP1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ULBP1 antibody and an ULBP1-HRP conjugate. The assay sample and buffer are incubated together with ULBP1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ULBP1 concentration since ULBP1 from samples and ULBP1-HRP conjugate compete for the anti-ULBP1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ULBP1 from the sample, fewer sites are left to bind ULBP1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ULBP1 concentration in each sample is interpolated from this standard curve.

119568161

EAW47776.1

27,997 Da

Adaptive Immune System Pathway (366160); Immune System Pathway (106386); Immunoregulatory Interactions Between A Lymphoid And A Non-Lymphoid Cell Pathway (106413); Natural Killer Cell Mediated Cytotoxicity Pathway (83079); Natural Killer Cell Mediated Cytotoxicity Pathway (490)

ULBP1: Ligand for the NKG2D receptor, together with at least ULBP2 and ULBP3. ULBPs activate multiple signaling pathways in primary NK cells, resulting in the production of cytokines and chemokines. Binding of ULBPs ligands to NKG2D induces calcium mobilization and activation of the JAK2, STAT5, ERK and PI3K kinase/Akt signal transduction pathway. In CMV infected cells, interacts with soluble CMV glycoprotein UL16. The interaction with UL16 blocked the interaction with the NKG2D receptor, providing a mechanism by which CMV infected cells might escape the immune system. UL16 also causes ULBP1 to be retained in the ER and cis- Golgi apparatus so that it does not reach the cell surface. Belongs to the MHC class I family. Protein type: Cell surface; Apoptosis; Membrane protein, GPI anchor. Chromosomal Location of Human Ortholog: 6q25. Cellular Component: anchored to plasma membrane; endoplasmic reticulum; plasma membrane. Molecular Function: peptide antigen binding; natural killer cell lectin-like receptor binding. Biological Process: regulation of immune response; antigen processing and presentation of peptide antigen via MHC class I; natural killer cell mediated cytotoxicity; natural killer cell activation

48-Strip-Wells

470 USD

96-Strip-Wells

675