ELISA/assay

Chicken Glutathione peroxidase ELISA Kit

MBS744245

48-Strip-Wells

470 USD

ELISA Kit

Chicken Glutathione peroxidase ELISA Kit

Glutathione peroxidase

glutathione peroxidase; Glutathione peroxidase 3; glutathione peroxidase 3; GPx-3; plasma glutathione peroxidase; extracellular glutathione peroxidase; glutathione peroxidase 3 (plasma); Extracellular glutathione peroxidase; Plasma glutathione peroxidase

GSH-Px

GPX3; GPX3; GPx-P; GSHPx-3; GSHPx-P; GPXP; GPx-3; GSHPx-3; GPx-P; GSHPx-P

GPX3_HUMAN

Chicken

Store all reagents at 2-8 degree C

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 ng/mL.

Intended Uses: This GSH-Px ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken GSH-Px. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Metabolism

Principle of the Assay: GSH-Px ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GSH-Px antibody and an GSH-Px-HRP conjugate. The assay sample and buffer are incubated together with GSH-Px-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GSH-Px concentration since GSH-Px from samples and GSH-Px-HRP conjugate compete for the anti-GSH-Px antibody binding site. Since the number of sites is limited, as more sites are occupied by GSH-Px from the sample, fewer sites are left to bind GSH-Px-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GSH-Px concentration in each sample is interpolated from this standard curve.

312004115

CAA41228.2

P22352

25,552 Da

Arachidonic Acid Metabolism Pathway (82991); Arachidonic Acid Metabolism Pathway (366); Folate Metabolism Pathway (198833); Glutathione Metabolism Pathway (82973); Glutathione Metabolism Pathway (198824); Glutathione Metabolism Pathway (343); Oxidative Stress Pathway (198916); Selenium Metabolism And Selenoproteins Pathway (198846); Selenium Pathway (198825); Glutathione Redox Reactions I Pathway (142227)

This gene product belongs to the glutathione peroxidase family, which functions in the detoxification of hydrogen peroxide. It contains a selenocysteine (Sec) residue at its active site. The selenocysteine is encoded by the UGA codon, which normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. [provided by RefSeq, Jul 2008]

GPX3: Protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione. Belongs to the glutathione peroxidase family. Protein type: Secreted; Lipid Metabolism - arachidonic acid; Secreted, signal peptide; Other Amino Acids Metabolism - glutathione; Oxidoreductase; EC 1.11.1.9. Chromosomal Location of Human Ortholog: 5q33.1. Cellular Component: extracellular space; extracellular region. Molecular Function: selenium binding; glutathione peroxidase activity; transcription factor binding. Biological Process: response to reactive oxygen species; hydrogen peroxide catabolic process; response to lipid hydroperoxide; protein homotetramerization

48-Strip-Wells

470 USD

96-Strip-Wells

675