ELISA/assay

Canine Lactoferrin ELISA Kit

MBS744201

48-Strip-Wells

470 USD

ELISA Kit

Canine Lactoferrin ELISA Kit

Lactoferrin

lactoferrin; Lactotransferrin; lactotransferrin; talalactoferrin; neutrophil lactoferrin; growth-inhibiting protein 12; epididymis luminal protein 110; lactotransferrin; Growth-inhibiting protein 12; TalalactoferrinCleaved into the following 5 chains:Lactoferricin-H; Lfcin-H; Kaliocin-1; Lactoferroxin-A; Lactoferroxin-B; Lactoferroxin-C

LF

LTF; LTF; LF; HLF2; GIG12; HEL110; GIG12; LF; Lactoferrin; Lfcin-H

TRFL_HUMAN

Canine

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

Intended Uses: This LF ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine LF. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Cardiovascular

Principle of the Assay: LF ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-LF antibody and an LF-HRP conjugate. The assay sample and buffer are incubated together with LF-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LF concentration since LF from samples and LF-HRP conjugate compete for the anti-LF antibody binding site. Since the number of sites is limited, as more sites are occupied by LF from the sample, fewer sites are left to bind LF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LF concentration in each sample is interpolated from this standard curve.

187122

AAA59511.1

73,161 Da

Amyloids Pathway (366238); Disease Pathway (530764); Latent Infection Of Homo Sapiens With Mycobacterium Tuberculosis Pathway (645267); Mtb Iron Assimilation By Chelation Pathway (645273); Phagosomal Maturation (early Endosomal Stage) Pathway (645268); Response Of Mtb To Phagocytosis Pathway (645269)

This gene is a member of the transferrin family of genes and its protein product is found in the secondary granules of neutrophils. The protein is a major iron-binding protein in milk and body secretions with an antimicrobial activity, making it an important component of the non-specific immune system. The protein demonstrates a broad spectrum of properties, including regulation of iron homeostasis, host defense against a broad range of microbial infections, anti-inflammatory activity, regulation of cellular growth and differentiation and protection against cancer development and metastasis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]

LTF: Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. Belongs to the transferrin family. 2 isoforms of the human protein are produced by alternative promoter. Protein type: Secreted; Protease; EC 3.4.21.-; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 3p21.31. Cellular Component: extracellular space; specific granule; cytoplasm; extracellular region; nucleus; secretory granule. Molecular Function: heparin binding; protein serine/threonine kinase activator activity; protein binding; DNA binding; ferric iron binding; serine-type endopeptidase activity; iron ion binding. Biological Process: negative regulation of lipopolysaccharide-mediated signaling pathway; interaction with host; ossification; positive regulation of I-kappaB kinase/NF-kappaB cascade; transcription, DNA-dependent; positive regulation of toll-like receptor 4 signaling pathway; antifungal humoral response; proteolysis; iron ion transport; humoral immune response; activation of NF-kappaB transcription factor; regulation of cytokine production; retinal homeostasis; positive regulation of osteoblast differentiation; innate immune response in mucosa; negative regulation of viral genome replication; response to host immune response; positive regulation of osteoblast proliferation; antibacterial humoral response; regulation of tumor necrosis factor production; negative regulation of viral reproduction; iron assimilation by chelation and transport; negative regulation of ATPase activity; negative regulation of apoptosis

48-Strip-Wells

470 USD

96-Strip-Wells

675