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ELISA/assay

Rat Tyrosine Hydroxylase ELISA Kit

MBS744156

96 Strip Wells

675 USD

MBS744156

ELISA Kit

Rat Tyrosine Hydroxylase ELISA Kit

Tyrosine Hydroxylase

tyrosine hydroxylase, partial; Tyrosine 3-monooxygenase; tyrosine 3-monooxygenase; dystonia 14; tyrosine 3-hydroxylase; tyrosine hydroxylase; Tyrosine 3-hydroxylase; TH

TH

TH; TH; TYH; DYT14; DYT5b; TYH; TH

TY3H_HUMAN

Rat

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Intended Uses: This TH ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat TH. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Sensitivity: The sensitivity in this assay is 0.1 ng/mL.

Neurobiology

Intended Uses: This TH ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat TH. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: TH ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TH antibody and an TH-HRP conjugate. The assay sample and buffer are incubated together with TH-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TH concentration since TH from samples and TH-HRP conjugate compete for the anti-TH antibody binding site. Since the number of sites is limited, as more sites are occupied by TH from the sample, fewer sites are left to bind TH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TH concentration in each sample is interpolated from this standard curve.

986909

AAA77650.1

44,898 Da

ATF-2 Transcription Factor Network Pathway 138006!!Alcoholism Pathway 585563!!Alcoholism Pathway 587116!!Alpha-synuclein Signaling Pathway 137913!!Amine-derived Hormones Pathway 160983!!Amphetamine Addiction Pathway 547607!!Amphetamine Addiction Pathway 550546!!Biogenic Amine Synthesis Pathway 198793!!Catecholamine Biosynthesis Pathway 160984!!Catecholamine Biosynthesis, Tyrosine = Dopamine = Noradrenaline = Adrenaline Pathway 413357

The protein encoded by this gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Jul 2008]

96 Strip Wells

675 USD