ELISA/assay

Monkey Cathepsin S ELISA Kit

MBS744105

48-Strip-Wells

470 USD

ELISA Kit

Monkey Cathepsin S ELISA Kit

Cathepsin S

cathepsin S; Cathepsin S; cathepsin S; cathepsin S

Cath-S

CTSS; CTSS

CATS_HUMAN

Monkey

This assay has high sensitivity and excellent specificity for detection of CTS. No significant cross-reactivity or interference between CTS and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CTS and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This CTS ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey CTS. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Immunology

. Principle of the assay: CTS ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CTS antibody and an CTS-HRP conjugate. The assay sample and buffer are incubated together with CTS-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CTS concentration since CTS from samples and CTS-HRP conjugate compete for the anti-CTS antibody binding site. Since the number of sites is limited, as more sites are occupied by CTS from the sample, fewer sites are left to bind CTS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CTS concentration in each sample is interpolated from this standard curve.

179957

AAC37592.1

31,626 Da

Adaptive Immune System Pathway (366160); Antigen Processing And Presentation Pathway (83074); Antigen Processing And Presentation Pathway (485); Antigen Processing-Cross Presentation Pathway (477122); Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway (730306); Class I MHC Mediated Antigen Processing Presentation Pathway (366161); Collagen Formation Pathway (645288); Degradation Of The Extracellular Matrix Pathway (576263); Endosomal/Vacuolar Pathway (477125); Extracellular Matrix Organization Pathway (576262)

The protein encoded by this gene, a member of the peptidase C1 family, is a lysosomal cysteine proteinase that may participate in the degradation of antigenic proteins to peptides for presentation on MHC class II molecules. The encoded protein can function as an elastase over a broad pH range in alveolar macrophages. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Dec 2010]

CTSS: Thiol protease. Key protease responsible for the removal of the invariant chain from MHC class II molecules. The bond- specificity of this proteinase is in part similar to the specificities of cathepsin L and cathepsin N. Belongs to the peptidase C1 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Protease; EC 3.4.22.27. Chromosomal Location of Human Ortholog: 1q21. Cellular Component: extracellular space; lysosomal lumen; membrane; intracellular membrane-bound organelle; lysosome; extracellular region. Molecular Function: collagen binding; proteoglycan binding; fibronectin binding; cysteine-type endopeptidase activity; laminin binding. Biological Process: adaptive immune response; extracellular matrix organization and biogenesis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent; antigen processing and presentation of exogenous peptide antigen via MHC class II; proteolysis; antigen processing and presentation; extracellular matrix disassembly; collagen catabolic process; antigen processing and presentation of peptide antigen via MHC class I; proteolysis involved in cellular protein catabolic process; toll-like receptor signaling pathway; innate immune response; antigen processing and presentation of exogenous peptide antigen via MHC class I; immune response; positive regulation of inflammatory response

48-Strip-Wells

470 USD

96-Strip-Wells

675