catalog number :
MBS744096
products type :
ELISA Kit
products full name :
Sheep Epinephrine/Adrenaline ELISA Kit
products short name :
[Epinephrine/Adrenaline]
other names :
[EPI protein; EPI protein; EPI protein]
products gene name :
[EPI]
uniprot entry name :
B9DR51_LEPMC
storage stability :
Store all reagents at 2-8 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, plasma, cell culture superna tants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.
products description :
For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate . INTENDED USE This EPI ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep EPI. This ELISA kit for research use only, not for therapeutic or diagnostic applications! . PRINCIPLE OF THE ASSAY EPI ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-EPI antibody and an EPI-HRP conjugate. The assay sample and buffer are incubated together with EPI-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensi ty ofcolor is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the EPI concentration since EPI from samples and EPI-HRP conjugate compete for the anti-EPI antibody binding site. Since the number of sites is limited, as more sites are occupied by EPI from the sample, fewer sites are left to bind EPI-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The EPI concentration in each sample is interpolated from this standard curve.
ncbi acc num :
CAQ03441.1
ncbi mol weight :
41,398 Da
size4 :
10x96-Strip-Wells