ELISA/assay

Sheep Growth Hormone ELISA Kit

MBS744003

48-Strip-Wells-(Competitive)

440 USD

ELISA Kit

Sheep Growth Hormone ELISA Kit

[Growth Hormone]

[growth hormone; Somatotropin; somatotropin; pituitary growth hormone; growth hormone 1; Growth hormone; GH; GH-N; Growth hormone 1; Pituitary growth hormone]

[GH]

[GH1; GH1; GH; GHN; GH-N; hGH-N; IGHD1B; GH; GH-N]

SOMA_HUMAN

Sheep

This assay has high sensitivity and excellent specificity for detection of GH. No significant cross-reactivity or interference between GH and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GH and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Quantitative Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This GH ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep GH. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: GH ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-GH antibody and an GH-HRP conjugate. The assay sample and buffer are incubated together with GH-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GH concentration since GH from samples and GH-HRP conjugate compete for the anti-GH antibody binding site. Since the number of sites is limited, as more sites are occupied by GH from the sample, fewer sites are left to bind GH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GH concentration in each sample is interpolated from this standard curve.

183157

AAA98618.1

20,201 Da

Adipogenesis Pathway (198832); Cytokine Signaling In Immune System Pathway (366171); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Endochondral Ossification Pathway (198812); Growth Hormone Receptor Signaling Pathway (477128); Immune System Pathway (106386); Jak-STAT Signaling Pathway (83077); Jak-STAT Signaling Pathway (488); Metabolism Of Proteins Pathway (106230)

The protein encoded by this gene is a member of the somatotropin/prolactin family of hormones which play an important role in growth control. The gene, along with four other related genes, is located at the growth hormone locus on chromosome 17 where they are interspersed in the same transcriptional orientation; an arrangement which is thought to have evolved by a series of gene duplications. The five genes share a remarkably high degree of sequence identity. Alternative splicing generates additional isoforms of each of the five growth hormones, leading to further diversity and potential for specialization. This particular family member is expressed in the pituitary but not in placental tissue as is the case for the other four genes in the growth hormone locus. Mutations in or deletions of the gene lead to growth hormone deficiency and short stature. [provided by RefSeq, Jul 2008]

GH: Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues. Defects in GH1 are a cause of growth hormone deficiency isolated type 1A (IGHD1A); also known as pituitary dwarfism I. IGHD1A is an autosomal recessive deficiency of GH which causes short stature. IGHD1A patients have an absence of GH with severe dwarfism and often develop anti-GH antibodies when given exogenous GH. Defects in GH1 are a cause of growth hormone deficiency isolated type 1B (IGHD1B); also known as dwarfism of Sindh. IGHD1B is an autosomal recessive deficiency of GH which causes short stature. IGHD1B patients have low but detectable levels of GH. Dwarfism is less severe than in IGHD1A and patients usually respond well to exogenous GH. Defects in GH1 are the cause of Kowarski syndrome (KWKS); also known as pituitary dwarfism VI. Defects in GH1 are a cause of growth hormone deficiency isolated type 2 (IGHD2). IGHD2 is an autosomal dominant deficiency of GH which causes short stature. Clinical severity is variable. Patients have a positive response and immunologic tolerance to growth hormone therapy. Belongs to the somatotropin/prolactin family. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Hormone; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 17q24.2. Cellular Component: extracellular space; extracellular region. Molecular Function: protein binding; growth hormone receptor binding; growth factor activity; prolactin receptor binding; hormone activity; metal ion binding. Biological Process: positive regulation of insulin-like growth factor receptor signaling pathway; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of MAP kinase activity; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of tyrosine phosphorylation of Stat5 protein; positive regulation of receptor internalization; positive regulation of JAK-STAT cascade; glucose transport; positive regulation of multicellular organism growth; JAK-STAT cascade; response to estradiol stimulus; positive regulation of tyrosine phosphorylation of Stat3 protein. Disease: Isolated Growth Hormone Deficiency, Type Ia; Isolated Growth Hormone Deficiency, Type Ib; Isolated Growth Hormone Deficiency, Type Ii; Kowarski Syndrome

48-Strip-Wells-(Competitive)

440 USD

48-Strip-Wells-(Sandwich)

440

96-Strip-Wells-(Competitive)

640

96-Strip-Wells-(Sandwich)

640

5x96-Strip-Wells-(Competitive)

2895

5x96-Strip-Wells-(Sandwich)

2895

10x96-Strip-Wells-(Competitive)

5415

10x96-Strip-Wells-(Sandwich)

5415