catalog number :
MBS743945
products type :
ELISA Kit
products full name :
Canine Mucin-5 subtype AC ELISA Kit
products short name :
Mucin-5 subtype AC
other names :
MUC5AC protein, partial; MUC5AC protein; MUC5AC protein
products gene name :
MUC5AC
other gene names :
MUC5AC
uniprot entry name :
Q96DU6_HUMAN
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.
other info2 :
Intended Uses: This MUC5AC ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine MUC5AC. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
products categories :
Signal Transduction
products description :
Principle of the Assay: MUC5AC ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MUC5AC antibody and an MUC5AC-HRP conjugate. The assay sample and buffer are incubated together with MUC5AC-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MUC5AC concentration since MUC5AC from samples and MUC5AC-HRP conjugate compete for the anti-MUC5AC antibody binding site. Since the number of sites is limited, as more sites are occupied by MUC5AC from the sample, fewer sites are left to bind MUC5AC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MUC5AC concentration in each sample is interpolated from this standard curve.
ncbi acc num :
CAC44892.1
ncbi mol weight :
6,501 Da