ELISA/assay

Sheep Myoglobin ELISA Kit

MBS743915

48-Strip-Wells

470 USD

ELISA Kit

Sheep Myoglobin ELISA Kit

Myoglobin

myoglobin; Myoglobin; myoglobin; myoglobin

Mb

MB; MB; PVALB; myoglobgin

MYG_HUMAN

Sheep

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive

Intended Uses: This MYO ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep MYO. This ELISA kit for research use only, not for therapeutic or diagnostic applications. !Intended Uses: This MYO ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep MYO. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Cardiovascular

Principle of the Assay: MYO ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MYO antibody and an MYO-HRP conjugate. The assay sample and buffer are incubated together with MYO-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MYO concentration since MYO from samples and MYO-HRP conjugate compete for the anti-MYO antibody binding site. Since the number of sites is limited, as more sites are occupied by MYO from the sample, fewer sites are left to bind MYO-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MYO concentration in each sample is interpolated from this standard curve.

44955888

NP_976312.1

NM_203378.1

17,184 Da

Effects Of Nitric Oxide Pathway (920994)

This gene encodes a member of the globin superfamily and is expressed in skeletal and cardiac muscles. The encoded protein is a haemoprotein contributing to intracellular oxygen storage and transcellular facilitated diffusion of oxygen. At least three alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq, Jul 2008]

MB: Serves as a reserve supply of oxygen and facilitates the movement of oxygen within muscles. Belongs to the globin family. Protein type: Carrier. Chromosomal Location of Human Ortholog: 22q13.1. Molecular Function: iron ion binding; heme binding; oxygen binding; oxygen transporter activity. Biological Process: slow-twitch skeletal muscle fiber contraction; response to hydrogen peroxide; oxygen transport; heart development; brown fat cell differentiation; response to hormone stimulus; response to hypoxia; enucleate erythrocyte differentiation

48-Strip-Wells

470 USD

96-Strip-Wells

675