ELISA/assay

Monkey Calcitonin ELISA Kit

MBS743907

48-Strip-Wells

470 USD

ELISA Kit

Monkey Calcitonin ELISA Kit

Calcitonin

calcitonin; Calcitonin; calcitonin; calcitonin gene-related peptide 1; katacalcin; calcitonin 1; alpha-type CGRP; calcitonin gene-related peptide I; calcitonin/calcitonin-related polypeptide, alpha; calcitonin-related polypeptide alpha; Calcitonin carboxyl-terminal peptide; CCP; PDN-21

CT

CALCA; CALCA; CT; KC; CGRP; CALC1; CGRP1; CGRP-I; CALC1; CCP

CALC_HUMAN

Monkey

This assay has high sensitivity and excellent specificity for detection of CT. No significant cross-reactivity or interference between CT and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CT and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

Intended Uses: This CT ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey CT. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Signal Transduction

Principle of the assay: CT ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CT antibody and an CT-HRP conjugate. The assay sample and buffer are incubated together with CT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CT concentration since CT from samples and CT-HRP conjugate compete for the anti-CT antibody binding site. Since the number of sites is limited, as more sites are occupied by CT from the sample, fewer sites are left to bind CT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CT concentration in each sample is interpolated from this standard curve.

179820

AAA58403.1

13,898 Da

Amyloids Pathway (366238); Calcitonin-like Ligand Receptors Pathway (106379); Class B/2 (Secretin Family Receptors) Pathway (106378); Disease Pathway (530764); Endothelin Pathway (672453); G Alpha (s) Signalling Events Pathway (119549); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); Myometrial Relaxation And Contraction Pathways (198759); Signal Transduction Pathway (477114)

This gene encodes the peptide hormones calcitonin, calcitonin gene-related peptide and katacalcin by tissue-specific alternative RNA splicing of the gene transcripts and cleavage of inactive precursor proteins. Calcitonin is involved in calcium regulation and acts to regulate phosphorus metabolism. Calcitonin gene-related peptide functions as a vasodilator and as an antimicrobial peptide while katacalcin is a calcium-lowering peptide. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2014]

CALCA: Calcitonin causes a rapid but short-lived drop in the level of calcium and phosphate in blood by promoting the incorporation of those ions in the bones. Belongs to the calcitonin family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 11p15.2. Cellular Component: extracellular space; cell soma; cytoplasm; extracellular region; terminal button; nucleus. Molecular Function: identical protein binding; protein binding; calcitonin receptor binding; hormone activity; protein complex binding; receptor binding. Biological Process: negative regulation of osteoclast differentiation; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); positive regulation of interleukin-1 alpha production; vasodilation of artery during baroreceptor response to increased systemic arterial blood pressure; response to pain; positive regulation of vasodilation; G-protein signaling, adenylate cyclase activating pathway; protein amino acid phosphorylation; positive regulation of interleukin-8 production; monocyte chemotaxis; leukocyte adhesion; elevation of cytosolic calcium ion concentration; cell-cell signaling; vasculature development; neuropeptide signaling pathway; negative regulation of smooth muscle contraction; regulation of blood pressure; receptor internalization; positive regulation of cAMP biosynthetic process; negative regulation of ossification; feeding behavior; positive regulation of adenylate cyclase activity; negative regulation of blood pressure; inflammatory response; positive regulation of macrophage differentiation; negative regulation of bone resorption; detection of temperature stimulus involved in sensory perception of pain; negative regulation of neurological process; aging; cytosolic calcium ion homeostasis; regulation of heart rate; adenylate cyclase activation; positive regulation of ossification; regulation of systemic arterial blood pressure by neurological process; G-protein coupled receptor internalization; response to heat; endothelial cell proliferation; regulation of inflammatory response; activation of protein kinase activity; endothelial cell migration; negative regulation of transcription, DNA-dependent; embryo implantation

48-Strip-Wells

470 USD

96-Strip-Wells

675