product summary
request information :
company name :
MyBioSource
product type :
ELISA/assay
product name :
Sheep Interleukin 8 ELISA Kit
catalog :
MBS743903
quantity :
96 Strip Wells
price :
675 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS743903
products type :
ELISA Kit
products full name :
Sheep Interleukin 8 ELISA Kit
products short name :
Interleukin 8
other names :
interleukin-8; Interleukin-8; interleukin-8; IL-8; C-X-C motif chemokine 8; chemokine (C-X-C motif) ligand 8; C-X-C motif chemokine 8; Chemokine (C-X-C motif) ligand 8
products gene name :
IL-8
other gene names :
CXCL8; CXCL8; IL8; IL8; IL-8
uniprot entry name :
IL8_MACMU
reactivity :
Sheep
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Intended Uses: This IL-8 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep IL-8. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
other info2 :
Sensitivity: The sensitivity in this assay is 1.0 pg/mL.
products categories :
Immunology
products description :
Principle of the Assay: IL-8 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IL-8 antibody and an IL-8-HRP conjugate. The assay sample and buffer are incubated together with IL-8-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL-8 concentration since IL-8 from samples and IL-8-HRP conjugate compete for the anti-IL-8 antibody binding site. Since the number of sites is limited, as more sites are occupied by IL-8 from the sample, fewer sites are left to bind IL-8-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL-8 concentration in each sample is interpolated from this standard curve.
ncbi gi num :
74136483
ncbi gb acc num :
NP_001028137.1
ncbi mol weight :
11,320 Da
ncbi pathways :
Amoebiasis Pathway 167336!!Amoebiasis Pathway 167191!!Bladder Cancer Pathway 86781!!Bladder Cancer Pathway 527!!Chagas Disease (American Trypanosomiasis) Pathway 147811!!Chagas Disease (American Trypanosomiasis) Pathway 147795!!Chemokine Signaling Pathway 99278!!Chemokine Signaling Pathway 96864!!Cytokine-cytokine Receptor Interaction Pathway 86721!!Cytokine-cytokine Receptor Interaction Pathway 460
size :
96 Strip Wells
price :
675 USD
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

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