ELISA/assay

Sheep Bone Morphogenetic Protein 2 ELISA Kit

MBS743900

48-Strip-Wells

470 USD

ELISA Kit

Sheep Bone Morphogenetic Protein 2 ELISA Kit

Bone Morphogenetic Protein 2

bone morphogenetic protein 2 preproprotein; Bone morphogenetic protein 2; bone morphogenetic protein 2; BMP-2A; bone morphogenetic protein 2A; bone morphogenetic protein 2; Bone morphogenetic protein 2A; BMP-2A

BMP2

BMP2; BMP2; BDA2; BMP2A; BMP2A; BMP-2; BMP-2A

BMP2_HUMAN

Sheep

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

Intended Uses: This BMP-2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep BMP-2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Cardiovascular

Principle of the Assay: BMP-2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-BMP-2 antibody and an BMP-2-HRP conjugate. The assay sample and buffer are incubated together with BMP-2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BMP-2 concentration since BMP-2 from samples and BMP-2-HRP conjugate compete for the anti-BMP-2 antibody binding site. Since the number of sites is limited, as more sites are occupied by BMP-2 from the sample, fewer sites are left to bind BMP-2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BMP-2 concentration in each sample is interpolated from this standard curve.

4557369

NP_001191.1

NM_001200.2

44,702 Da

Adipogenesis Pathway (198832); BDNF Signaling Pathway (712093); BMP Signalling And Regulation Pathway (198910); Basal Cell Carcinoma Pathway (83113); Basal Cell Carcinoma Pathway (525); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Elastic Fibre Formation Pathway (730310); Extracellular Matrix Organization Pathway (576262); Heart Development Pathway (198802)

The protein encoded by this gene belongs to the transforming growth factor-beta (TGFB) superfamily. The encoded protein acts as a disulfide-linked homodimer and induces bone and cartilage formation. [provided by RefSeq, Jul 2008]

48-Strip-Wells

470 USD

96-Strip-Wells

675