ELISA/assay

Monkey Deoxypyridinoline ELISA Kit

MBS743890

48-Strip-Wells

470 USD

ELISA Kit

Monkey Deoxypyridinoline ELISA Kit

Deoxypyridinoline

DPD

Monkey

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 nmol/L.

Intended Uses: This DPD ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey DPD. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: DPD ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-DPD antibody and an DPD-HRP conjugate. The assay sample and buffer are incubated together with DPD-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DPD concentration since DPD from samples and DPD-HRP conjugate compete for the anti-DPD antibody binding site. Since the number of sites is limited, as more sites are occupied by DPD from the sample, fewer sites are left to bind DPD-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DPD concentration in each sample is interpolated from this standard curve.

48-Strip-Wells

470 USD

96-Strip-Wells

675