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ELISA/assay

Canine Plasminogen Activator Inhibitor 1 ELISA Kit

MBS743848

96 Strip Wells

675 USD

MBS743848

ELISA Kit

Canine Plasminogen Activator Inhibitor 1 ELISA Kit

Plasminogen Activator Inhibitor 1

plasminogen activator inhibitor 1; Plasminogen activator inhibitor 1; plasminogen activator inhibitor 1; serpin E1; endothelial plasminogen activator inhibitor; serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1; serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1; Endothelial plasminogen activator inhibitor; Serpin E1

PAI 1

SERPINE1; SERPINE1; PAI; PAI1; PAI-1; PLANH1; PAI1; PLANH1; PAI; PAI-1

PAI1_HUMAN

Canine

This assay has high sensitivity and excellent specificity for detection of PAI-1. No significant cross-reactivity or interference between PAI-1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PAI-1 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Intended Uses: This PAI-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine PAI-1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Sensitivity: The sensitivity in this assay is 1.0 pg/mL.

Cardiovascular

Principle of the Assay: PAI-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PAI-1 antibody and an PAI-1-HRP conjugate. The assay sample and buffer are incubated together with PAI-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PAI-1 concentration since PAI-1 from samples and PAI-1-HRP conjugate compete for the anti-PAI-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by PAI-1 from the sample, fewer sites are left to bind PAI-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PAI-1 concentration in each sample is interpolated from this standard curve.

10835159

NP_000593.1

NM_000602.4

43,404 Da

Adipogenesis Pathway 198832!!BMAL1:CLOCK,NPAS2 Activates Circadian Gene Expression Pathway 477138!!Blood Clotting Cascade Pathway 198840!!Chagas Disease (American Trypanosomiasis) Pathway 147809!!Chagas Disease (American Trypanosomiasis) Pathway 147795!!Circadian Clock Pathway 187173!!Complement And Coagulation Cascades Pathway 198880!!Complement And Coagulation Cascades Pathway 83073!!Complement And Coagulation Cascades Pathway 484!!Direct P53 Effectors Pathway 137939

This gene encodes a member of the serine proteinase inhibitor (serpin) superfamily. This member is the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), and hence is an inhibitor of fibrinolysis. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]

96 Strip Wells

675 USD