Canine Insulin Like Growth Factor Binding Protein 3 ELISA Kit | MyBioSource MBS743827 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Canine Insulin Like Growth Factor Binding Protein 3 ELISA Kit

catalog :

MBS743827

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS743827

products type :

ELISA Kit

products full name :

Canine Insulin Like Growth Factor Binding Protein 3 ELISA Kit

products short name :

Insulin Like Growth Factor Binding Protein 3

other names :

insulin-like growth factor-binding protein 3; Insulin-like growth factor-binding protein 3; insulin-like growth factor-binding protein 3; IBP-3; IGFBP-3; IGF-binding protein 3; insulin-like growth factor-binding protein (IGF-BP3); insulin-like growth factor binding protein 3

products gene name :

IGFBP3

other gene names :

Igfbp3; Igfbp3; IGF-BP3; Igfbp-3; IBP-3; IGF-binding protein 3; IGFBP-3

uniprot entry name :

IBP3_RAT

reactivity :

Canine

specificity :

This assay has high sensitivity and excellent specificity for detection of IGFBP-3. No significant cross-reactivity or interference between IGFBP-3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IGFBP-3 and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Quantitative Competitive. Sensitivity: 0.1 ug/mL.

products categories :

Cell Biology

products description :

Intended Uses: This IGFBP-3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine IGFBP-3. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: IGFBP-3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IGFBP-3 antibody and an IGFBP-3-HRP conjugate. The assay sample and buffer are incubated together with IGFBP-3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IGFBP-3 concentration since IGFBP-3 from samples and IGFBP-3-HRP conjugate compete for the anti-IGFBP-3 antibody binding site. Since the number of sites is limited, as more sites are occupied by IGFBP-3 from the sample, fewer sites are left to bind IGFBP-3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IGFBP-3 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

298231182

ncbi acc num :

NP_036720.2

ncbi gb acc num :

NM_012588.2

ncbi mol weight :

31,680 Da

ncbi pathways :

Myometrial Relaxation And Contraction Pathways (198465); Transcriptional Misregulation In Cancer Pathway (523027); Transcriptional Misregulation In Cancer Pathway (522987); P53 Pathway (219776); P53 Signal Pathway (198463); P53 Signaling Pathway (83447); P53 Signaling Pathway (465)

ncbi summary :

an insulin growth factor (Igf) binding protein; involved in modulating IGF-I action [RGD, Feb 2006]

uniprot summary :

IGFBP3: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R. Protein type: Secreted, signal peptide; Cell development/differentiation; Secreted. Cellular Component: extracellular space; nucleus. Molecular Function: insulin-like growth factor binding; protein binding; insulin-like growth factor I binding; fibronectin binding; insulin-like growth factor II binding; protein tyrosine phosphatase activator activity. Biological Process: positive regulation of catalytic activity; positive regulation of insulin-like growth factor receptor signaling pathway; regulation of insulin-like growth factor receptor signaling pathway; negative regulation of smooth muscle cell proliferation; positive regulation of apoptosis; negative regulation of smooth muscle cell migration; protein amino acid phosphorylation; positive regulation of myoblast differentiation; osteoblast differentiation; negative regulation of cell proliferation; negative regulation of protein amino acid phosphorylation; positive regulation of MAPKKK cascade; regulation of growth; regulation of cell growth

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage