ELISA/assay

Goat Glucagon Like Peptide 2 ELISA Kit

MBS743757

48-Strip-Wells

470 USD

ELISA Kit

Goat Glucagon Like Peptide 2 ELISA Kit

Glucagon Like Peptide 2

glucagon preproprotein; Glucagon; glucagon; preproglucagon; glucagon-like peptide 1; glucagon-like peptide 2; glicentin-related polypeptide; glucagon; Incretin hormone

GLP-2

GCG; GCG; GLP1; GLP2; GRPP; GRPP; OXM; OXY; GLP-1; GLP-1(7-37); GLP-1(7-36); GLP-2

GLUC_HUMAN

Goat

This assay has high sensitivity and excellent specificity for detection of GLP-2. No significant cross-reactivity or interference between GLP-2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GLP-2 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This GLP-2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Goat GLP-2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Signal Transduction

Principle of the assay: GLP-2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GLP-2 antibody and an GLP-2-HRP conjugate. The assay sample and buffer are incubated together with GLP-2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GLP-2 concentration since GLP-2 from samples and GLP-2-HRP conjugate compete for the anti-GLP-2 antibody binding site. Since the number of sites is limited, as more sites are occupied by GLP-2 from the sample, fewer sites are left to bind GLP-2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GLP-2 concentration in each sample is interpolated from this standard curve.

4503945

NP_002045.1

NM_002054.4

20,909 Da

Class B/2 (Secretin Family Receptors) Pathway (106378); FOXA1 Transcription Factor Network Pathway (137979); G Alpha (q) Signalling Events Pathway (106043); G Alpha (s) Signalling Events Pathway (119549); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); Gastrin-CREB Signalling Pathway Via PKC And MAPK (645295); Glucagon Signaling In Metabolic Regulation Pathway (106097); Glucagon-like Peptide-1 (GLP1) Regulates Insulin Secretion Pathway (105925); Glucagon-type Ligand Receptors Pathway (106380)

The protein encoded by this gene is actually a preproprotein that is cleaved into four distinct mature peptides. One of these, glucagon, is a pancreatic hormone that counteracts the glucose-lowering action of insulin by stimulating glycogenolysis and gluconeogenesis. Glucagon is a ligand for a specific G-protein linked receptor whose signalling pathway controls cell proliferation. Two of the other peptides are secreted from gut endocrine cells and promote nutrient absorption through distinct mechanisms. Finally, the fourth peptide is similar to glicentin, an active enteroglucagon. [provided by RefSeq, Jul 2008]

GCG: Glucagon plays a key role in glucose metabolism and homeostasis. Regulates blood glucose by increasing gluconeogenesis and decreasing glycolysis. A counterregulatory hormone of insulin, raises plasma glucose levels in response to insulin-induced hypoglycemia. Plays an important role in initiating and maintaining hyperglycemic conditions in diabetes. Glucagon release is stimulated by hypoglycemia and inhibited by hyperglycemia, insulin, and somatostatin. GLP-1 and GLP-2 are induced in response to nutrient ingestion. Glucagon is secreted in the A cells of the islets of Langerhans. GLP-1, GLP-2, oxyntomodulin and glicentin are secreted from enteroendocrine cells throughout the gastrointestinal tract. GLP1 and GLP2 are also secreted in selected neurons in the brain. Belongs to the glucagon family. Protein type: Secreted; Secreted, signal peptide; Hormone. Chromosomal Location of Human Ortholog: 2q36-q37. Cellular Component: extracellular space; endoplasmic reticulum lumen; extracellular region; plasma membrane. Molecular Function: identical protein binding; protein binding; hormone activity; glucagon receptor binding; receptor binding. Biological Process: positive regulation of protein binding; positive regulation of histone H3-K4 methylation; signal transduction; positive regulation of peptidyl-serine phosphorylation; response to starvation; G-protein signaling, coupled to cAMP nucleotide second messenger; G-protein coupled receptor protein signaling pathway; cell proliferation; cellular protein metabolic process; positive regulation of protein kinase activity; positive regulation of cAMP biosynthetic process; energy reserve metabolic process; feeding behavior; regulation of insulin secretion; negative regulation of apoptosis

48-Strip-Wells

470 USD

96-Strip-Wells

675